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Biosensor Cell-Fit-HD(4D) for correlation of single-cell fate and microscale energy deposition in complex ion beams

We present a protocol for the biosensor Cell-Fit-HD(4D). It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registr...

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Detalles Bibliográficos
Autores principales: Schlegel, Julian, Liew, Hans, Rein, Katrin, Dzyubachyk, Oleh, Debus, Jürgen, Abdollahi, Amir, Niklas, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627659/
https://www.ncbi.nlm.nih.gov/pubmed/36340882
http://dx.doi.org/10.1016/j.xpro.2022.101798