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3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite sca...

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Autores principales: Duan, Jiahui, Cao, Yanyan, Shen, Zhizhong, Cheng, Yongqiang, Ma, Zhuwei, Wang, Lijing, Zhang, Yating, An, Yuchuan, Sang, Shengbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628872/
https://www.ncbi.nlm.nih.gov/pubmed/35058399
http://dx.doi.org/10.4014/jmb.2111.11003
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author Duan, Jiahui
Cao, Yanyan
Shen, Zhizhong
Cheng, Yongqiang
Ma, Zhuwei
Wang, Lijing
Zhang, Yating
An, Yuchuan
Sang, Shengbo
author_facet Duan, Jiahui
Cao, Yanyan
Shen, Zhizhong
Cheng, Yongqiang
Ma, Zhuwei
Wang, Lijing
Zhang, Yating
An, Yuchuan
Sang, Shengbo
author_sort Duan, Jiahui
collection PubMed
description Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.
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spelling pubmed-96288722022-12-13 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma Duan, Jiahui Cao, Yanyan Shen, Zhizhong Cheng, Yongqiang Ma, Zhuwei Wang, Lijing Zhang, Yating An, Yuchuan Sang, Shengbo J Microbiol Biotechnol Research article Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug. The Korean Society for Microbiology and Biotechnology 2022-04-28 2022-01-13 /pmc/articles/PMC9628872/ /pubmed/35058399 http://dx.doi.org/10.4014/jmb.2111.11003 Text en Copyright © 2022 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Duan, Jiahui
Cao, Yanyan
Shen, Zhizhong
Cheng, Yongqiang
Ma, Zhuwei
Wang, Lijing
Zhang, Yating
An, Yuchuan
Sang, Shengbo
3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title_full 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title_fullStr 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title_full_unstemmed 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title_short 3D Bioprinted GelMA/PEGDA Hybrid Scaffold for Establishing an In Vitro Model of Melanoma
title_sort 3d bioprinted gelma/pegda hybrid scaffold for establishing an in vitro model of melanoma
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628872/
https://www.ncbi.nlm.nih.gov/pubmed/35058399
http://dx.doi.org/10.4014/jmb.2111.11003
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