Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom

A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4(T), was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4(T) was most closely related to Caenimonas terrae SGM1-15(T) and Caenimonas koreensis EMB320(T) with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4(T) and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C(16:0), cyclo C(17:0), summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), and summed feature 8 (C(18:1)ω7c and/or C(18:1)ω6c). Strain DR4-4(T) possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4(T) represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4(T) (=KCTC 82470(T) =JCM 34453(T)).