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Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215

Gamma-aminobutyric acid (GABA) improves various physiological illnesses, including diabetes, hypertension, depression, memory lapse, and insomnia in humans. Therefore, interest in the commercial production of GABA is steadily increasing. Lactic acid bacteria (LAB) have widely been reported as a GABA...

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Autores principales: Kim, Jaegon, Lee, Myung-Hyun, Kim, Min-Sun, Kim, Gyeong-Hwuii, Yoon, Sung-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628908/
https://www.ncbi.nlm.nih.gov/pubmed/35586927
http://dx.doi.org/10.4014/jmb.2204.04029
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author Kim, Jaegon
Lee, Myung-Hyun
Kim, Min-Sun
Kim, Gyeong-Hwuii
Yoon, Sung-Sik
author_facet Kim, Jaegon
Lee, Myung-Hyun
Kim, Min-Sun
Kim, Gyeong-Hwuii
Yoon, Sung-Sik
author_sort Kim, Jaegon
collection PubMed
description Gamma-aminobutyric acid (GABA) improves various physiological illnesses, including diabetes, hypertension, depression, memory lapse, and insomnia in humans. Therefore, interest in the commercial production of GABA is steadily increasing. Lactic acid bacteria (LAB) have widely been reported as a GABA producer and are safe for human consumption. In this study, GABA-producing LAB were preliminarily identified and quantified via GABase assay. The acid and bile tolerance of the L. plantarum FBT215 strain were evaluated. The one-factor-at-a-time (OFAT) strategy was applied to determine the optimal conditions for GABA production using HPLC. Response surface methodology (RSM) with Box-Behnken design was used to predict the optimum GABA production. The strain FBT215 was shown to be acid and bile tolerant. The optimization of GABA production via the OFAT strategy resulted in an average GABA concentration of 1688.65 ± 14.29 μg/ml, while it was 1812.16 ± 23.16 μg/ml when RSM was applied. In conclusion, this study provides the optimum culture conditions for GABA production by the strain FBT215 and indicates that L. plantarum FBT215 is potentially promising for commercial functional probiotics with health claims.
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spelling pubmed-96289082022-12-13 Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215 Kim, Jaegon Lee, Myung-Hyun Kim, Min-Sun Kim, Gyeong-Hwuii Yoon, Sung-Sik J Microbiol Biotechnol Research article Gamma-aminobutyric acid (GABA) improves various physiological illnesses, including diabetes, hypertension, depression, memory lapse, and insomnia in humans. Therefore, interest in the commercial production of GABA is steadily increasing. Lactic acid bacteria (LAB) have widely been reported as a GABA producer and are safe for human consumption. In this study, GABA-producing LAB were preliminarily identified and quantified via GABase assay. The acid and bile tolerance of the L. plantarum FBT215 strain were evaluated. The one-factor-at-a-time (OFAT) strategy was applied to determine the optimal conditions for GABA production using HPLC. Response surface methodology (RSM) with Box-Behnken design was used to predict the optimum GABA production. The strain FBT215 was shown to be acid and bile tolerant. The optimization of GABA production via the OFAT strategy resulted in an average GABA concentration of 1688.65 ± 14.29 μg/ml, while it was 1812.16 ± 23.16 μg/ml when RSM was applied. In conclusion, this study provides the optimum culture conditions for GABA production by the strain FBT215 and indicates that L. plantarum FBT215 is potentially promising for commercial functional probiotics with health claims. The Korean Society for Microbiology and Biotechnology 2022-06-28 2022-05-13 /pmc/articles/PMC9628908/ /pubmed/35586927 http://dx.doi.org/10.4014/jmb.2204.04029 Text en Copyright © 2022 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Kim, Jaegon
Lee, Myung-Hyun
Kim, Min-Sun
Kim, Gyeong-Hwuii
Yoon, Sung-Sik
Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title_full Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title_fullStr Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title_full_unstemmed Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title_short Probiotic Properties and Optimization of Gamma-Aminobutyric Acid Production by Lactiplantibacillus plantarum FBT215
title_sort probiotic properties and optimization of gamma-aminobutyric acid production by lactiplantibacillus plantarum fbt215
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628908/
https://www.ncbi.nlm.nih.gov/pubmed/35586927
http://dx.doi.org/10.4014/jmb.2204.04029
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