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Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis

Proteins related to DNA replication have been proposed as cancer biomarkers and targets for anticancer agents. Among them, minichromosome maintenance (MCM) proteins, often overexpressed in various cancer cells, are recognized both as notable biomarkers for cancer diagnosis and as targets for cancer...

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Autores principales: Yun, Hee Jung, Jeoung, Da Jeoung, Jin, Soojung, Park, Jung-ha, Lee, Eun-Woo, Lee, Hyun-Tai, Choi, Yung Hyun, Kim, Byung Woo, Kwon, Hyun Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628924/
https://www.ncbi.nlm.nih.gov/pubmed/35880481
http://dx.doi.org/10.4014/jmb.2205.05012
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author Yun, Hee Jung
Jeoung, Da Jeoung
Jin, Soojung
Park, Jung-ha
Lee, Eun-Woo
Lee, Hyun-Tai
Choi, Yung Hyun
Kim, Byung Woo
Kwon, Hyun Ju
author_facet Yun, Hee Jung
Jeoung, Da Jeoung
Jin, Soojung
Park, Jung-ha
Lee, Eun-Woo
Lee, Hyun-Tai
Choi, Yung Hyun
Kim, Byung Woo
Kwon, Hyun Ju
author_sort Yun, Hee Jung
collection PubMed
description Proteins related to DNA replication have been proposed as cancer biomarkers and targets for anticancer agents. Among them, minichromosome maintenance (MCM) proteins, often overexpressed in various cancer cells, are recognized both as notable biomarkers for cancer diagnosis and as targets for cancer treatment. Here, we investigated the activity of cedrol, a single compound isolated from Juniperus chinensis, in reducing the expression of MCM proteins in human lung carcinoma A549 cells. Remarkably, cedrol also strongly inhibited the expression of all other MCM protein family members in A549 cells. Moreover, cedrol treatment reduced cell viability in A549 cells, accompanied by cell cycle arrest at the G1 phase, and enhanced apoptosis. Taken together, this study broadens our understanding of how cedrol executes its anticancer activity while demonstrating that cedrol has potential application in the treatment of human lung cancer as an inhibitor of MCM proteins.
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spelling pubmed-96289242022-12-13 Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis Yun, Hee Jung Jeoung, Da Jeoung Jin, Soojung Park, Jung-ha Lee, Eun-Woo Lee, Hyun-Tai Choi, Yung Hyun Kim, Byung Woo Kwon, Hyun Ju J Microbiol Biotechnol Research article Proteins related to DNA replication have been proposed as cancer biomarkers and targets for anticancer agents. Among them, minichromosome maintenance (MCM) proteins, often overexpressed in various cancer cells, are recognized both as notable biomarkers for cancer diagnosis and as targets for cancer treatment. Here, we investigated the activity of cedrol, a single compound isolated from Juniperus chinensis, in reducing the expression of MCM proteins in human lung carcinoma A549 cells. Remarkably, cedrol also strongly inhibited the expression of all other MCM protein family members in A549 cells. Moreover, cedrol treatment reduced cell viability in A549 cells, accompanied by cell cycle arrest at the G1 phase, and enhanced apoptosis. Taken together, this study broadens our understanding of how cedrol executes its anticancer activity while demonstrating that cedrol has potential application in the treatment of human lung cancer as an inhibitor of MCM proteins. The Korean Society for Microbiology and Biotechnology 2022-07-28 2022-07-01 /pmc/articles/PMC9628924/ /pubmed/35880481 http://dx.doi.org/10.4014/jmb.2205.05012 Text en Copyright © 2022 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Yun, Hee Jung
Jeoung, Da Jeoung
Jin, Soojung
Park, Jung-ha
Lee, Eun-Woo
Lee, Hyun-Tai
Choi, Yung Hyun
Kim, Byung Woo
Kwon, Hyun Ju
Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title_full Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title_fullStr Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title_full_unstemmed Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title_short Induction of Cell Cycle Arrest, Apoptosis, and Reducing the Expression of MCM Proteins in Human Lung Carcinoma A549 Cells by Cedrol, Isolated from Juniperus chinensis
title_sort induction of cell cycle arrest, apoptosis, and reducing the expression of mcm proteins in human lung carcinoma a549 cells by cedrol, isolated from juniperus chinensis
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628924/
https://www.ncbi.nlm.nih.gov/pubmed/35880481
http://dx.doi.org/10.4014/jmb.2205.05012
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