Cargando…

Novel High-Throughput DNA Part Characterization Technique for Synthetic Biology

This study presents a novel DNA part characterization technique that increases throughput by combinatorial DNA part assembly, solid plate-based quantitative fluorescence assay for phenotyping, and barcode tagging-based long-read sequencing for genotyping. We confirmed that the fluorescence intensiti...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bak, Seong-Kun, Seong, Wonjae, Rha, Eugene, Lee, Hyewon, Kim, Seong Keun, Kwon, Kil Koang, Kim, Haseong, Lee, Seung-Goo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Korean Society for Microbiology and Biotechnology 2022
Materias:	Research article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628936/ https://www.ncbi.nlm.nih.gov/pubmed/35879270 http://dx.doi.org/10.4014/jmb.2207.07013

Ejemplares similares

A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
por: Yeom, Soo-Jin, et al.
Publicado: (2018)

CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
por: Kim, Seong Keun, et al.
Publicado: (2022)

Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system
por: Kwon, Kil Koang, et al.
Publicado: (2020)

Antagonistic Control of Genetic Circuit Performance for Rapid Analysis of Targeted Enzyme Activity in Living Cells
por: Kwon, Kil Koang, et al.
Publicado: (2021)

Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
por: Yeom, Soo-Jin, et al.
Publicado: (2018)

A Genetically Encoded Biosensor for the Detection of Levulinic Acid
por: Kim, Tae Hyun, et al.
Publicado: (2023)

Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay
por: Lee, Hyewon, et al.
Publicado: (2020)

CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440
por: Kim, Seong Keun, et al.
Publicado: (2019)

Controlled Aggregation and Increased Stability of β-Glucuronidase by Cellulose Binding Domain Fusion
por: Yeom, Soo-Jin, et al.
Publicado: (2017)

C1 Compound Biosensors: Design, Functional Study, and Applications
por: Lee, Jin-Young, et al.
Publicado: (2019)

Evolution of enzymes with new specificity by high-throughput screening using DmpR-based genetic circuits and multiple flow cytometry rounds
por: Kwon, Kil Koang, et al.
Publicado: (2018)

Complete Genome Sequence of the Soil Bacterium Pseudomonas kribbensis Strain 46-2(T)
por: Jeong, Haeyoung, et al.
Publicado: (2018)

CRISPR interference-guided multiplex repression of endogenous competing pathway genes for redirecting metabolic flux in Escherichia coli
por: Kim, Seong Keun, et al.
Publicado: (2017)

Long-Term Stable and Tightly Controlled Expression of Recombinant Proteins in Antibiotics-Free Conditions
por: Yeom, Soo-Jin, et al.
Publicado: (2016)

Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus
por: Lee, Jin-Young, et al.
Publicado: (2020)

The Clinical Features and Risk Factors of Subglottic Cysts in Children: A Clinical Experience Using the Spontaneous Respiration Technique
por: Han, Seung Hoon, et al.
Publicado: (2023)

A molecular nanodevice for targeted degradation of mRNA during protein synthesis
por: Lee, Kyung-Ho, et al.
Publicado: (2016)

Genetically Encoded Biosensor-Based Screening for Directed Bacteriophage T4 Lysozyme Evolution
por: Woo, Seung-Gyun, et al.
Publicado: (2020)

In-house development of an optimized synthetic module for routine [(11)C]acetate production
por: Jang, Hwa Youn, et al.
Publicado: (2015)

Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus
por: Le, Thien-Kim, et al.
Publicado: (2021)

Feasibility of Surgical Treatment for Laryngomalacia Using the Spontaneous Respiration Technique
por: Cha, Hyunkyung, et al.
Publicado: (2021)

Synthetic Biology Tools for Genome and Transcriptome Engineering of Solventogenic Clostridium
por: Kwon, Seong Woo, et al.
Publicado: (2020)

High-Throughput Phenotypic Characterization and Diversity Analysis of Soybean Roots (Glycine max L.)
por: Kim, Seong-Hoon, et al.
Publicado: (2022)

BioPartsBuilder: a synthetic biology tool for combinatorial assembly of biological parts
por: Yang, Kun, et al.
Publicado: (2016)

Development of a Novel Short Synthetic Antibacterial Peptide Derived from the Swallowtail Butterfly Papilio xuthus Larvae
por: Kim, Seong Ryul, et al.
Publicado: (2020)

Lung cancer with superior vena cava syndrome diagnosed by intravascular biopsy using EBUS-TBNA
por: Lee, Daegeun, et al.
Publicado: (2016)

Recent Trends in Transurethral Surgeries and Urological Outpatient Procedures: a Nationwide Population-based Cohort Study
por: Kim, Jong Keun, et al.
Publicado: (2020)

Predictors and prognostic impact of post-operative atrial fibrillation in patients with hip fracture surgery
por: Bae, Seong Jun, et al.
Publicado: (2022)

Assessment of Esophageal Reconstruction via Bioreactor Cultivation of a Synthetic Scaffold in a Canine Model
por: Kim, In Gul, et al.
Publicado: (2023)

Tapered Waist Tensile Specimens for Evaluating Butt Fusion Joints of Polyethylene Pipes—Part 1: Development
por: Kim, Sunwoo, et al.
Publicado: (2022)

Prevalence of Disc Degeneration in Asymptomatic Korean Subjects. Part 3 : Cervical and Lumbar Relationship
por: Kim, Sang Jin, et al.
Publicado: (2013)

Correction of the Electrical and Thermal Extrinsic Effects in Thermoelectric Measurements by the Harman Method
por: Kang, Min-Su, et al.
Publicado: (2016)

Structural roles of guide RNAs in the nuclease activity of Cas9 endonuclease
por: Lim, Youngbin, et al.
Publicado: (2016)

Integrating Text-Mining and Balanced Scorecard Techniques to Investigate the Association between CEO Message of Homepage Words and Financial Status: Emphasis on Hospitals
por: Na, Hyung Jong, et al.
Publicado: (2021)

Tomographic and histometric analysis of autogenous bone block and synthetic hydroxyapatite block grafts without rigid fixation on rabbit calvaria
por: Bae, Soo-Yong, et al.
Publicado: (2014)

High-throughput phenotyping platform for analyzing drought tolerance in rice
por: Kim, Song Lim, et al.
Publicado: (2020)

Harman Measurements for Thermoelectric Materials and Modules under Non-Adiabatic Conditions
por: Roh, Im-Jun, et al.
Publicado: (2016)

Biological Parts for Kluyveromyces marxianus Synthetic Biology
por: Rajkumar, Arun S., et al.
Publicado: (2019)

Endoscopic management of pin stuck into the segmental bronchus
por: Kim, Young Chul, et al.
Publicado: (2021)

Evaluation of Contact Heat Transfer Coefficient and Phase Transformation during Hot Stamping of a Hat-Type Part
por: Kim, Heung-Kyu, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_mbi0kfq5sagtmbmb03ahthbhsm