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Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses
Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Society for Microbiology and Biotechnology
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628956/ https://www.ncbi.nlm.nih.gov/pubmed/35879284 http://dx.doi.org/10.4014/jmb.2204.04009 |
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author | Lee, Imchang Podolich, Olga Brenig, Bertram Tiwari, Sandeep Azevedo, Vasco de Carvalho, Daniel Santana Uetanabaro, Ana Paula Trovatti Góes-Neto, Aristóteles Alzahrani, Khalid J. Reva, Oleg Kozyrovska, Natalia de Vera, Jean-Pierre Barh, Debmalya Kim, Bong-Soo |
author_facet | Lee, Imchang Podolich, Olga Brenig, Bertram Tiwari, Sandeep Azevedo, Vasco de Carvalho, Daniel Santana Uetanabaro, Ana Paula Trovatti Góes-Neto, Aristóteles Alzahrani, Khalid J. Reva, Oleg Kozyrovska, Natalia de Vera, Jean-Pierre Barh, Debmalya Kim, Bong-Soo |
author_sort | Lee, Imchang |
collection | PubMed |
description | Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers. |
format | Online Article Text |
id | pubmed-9628956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-96289562022-12-13 Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses Lee, Imchang Podolich, Olga Brenig, Bertram Tiwari, Sandeep Azevedo, Vasco de Carvalho, Daniel Santana Uetanabaro, Ana Paula Trovatti Góes-Neto, Aristóteles Alzahrani, Khalid J. Reva, Oleg Kozyrovska, Natalia de Vera, Jean-Pierre Barh, Debmalya Kim, Bong-Soo J Microbiol Biotechnol Research article Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers. The Korean Society for Microbiology and Biotechnology 2022-08-28 2022-07-07 /pmc/articles/PMC9628956/ /pubmed/35879284 http://dx.doi.org/10.4014/jmb.2204.04009 Text en Copyright © 2022 by the authors. Licensee KMB. https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research article Lee, Imchang Podolich, Olga Brenig, Bertram Tiwari, Sandeep Azevedo, Vasco de Carvalho, Daniel Santana Uetanabaro, Ana Paula Trovatti Góes-Neto, Aristóteles Alzahrani, Khalid J. Reva, Oleg Kozyrovska, Natalia de Vera, Jean-Pierre Barh, Debmalya Kim, Bong-Soo Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title | Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title_full | Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title_fullStr | Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title_full_unstemmed | Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title_short | Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses |
title_sort | metagenome-assembled genomes of komagataeibacter from kombucha exposed to mars-like conditions reveal the secrets in tolerating extraterrestrial stresses |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628956/ https://www.ncbi.nlm.nih.gov/pubmed/35879284 http://dx.doi.org/10.4014/jmb.2204.04009 |
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