Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay

A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corres...

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Autores principales: Maksyutov, Rinat A., Gavrilova, Elena V., Shchelkunov, Sergei N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629046/
https://www.ncbi.nlm.nih.gov/pubmed/27477914
http://dx.doi.org/10.1016/j.jviromet.2016.07.024
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author Maksyutov, Rinat A.
Gavrilova, Elena V.
Shchelkunov, Sergei N.
author_facet Maksyutov, Rinat A.
Gavrilova, Elena V.
Shchelkunov, Sergei N.
author_sort Maksyutov, Rinat A.
collection PubMed
description A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species.
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spelling pubmed-96290462022-11-03 Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay Maksyutov, Rinat A. Gavrilova, Elena V. Shchelkunov, Sergei N. J Virol Methods Article A method of one-stage rapid detection and differentiation of epidemiologically important variola virus (VARV), monkeypox virus (MPXV), and varicella-zoster virus (VZV) utilizing multiplex real-time TaqMan PCR assay was developed. Four hybridization probes with various fluorescent dyes and the corresponding fluorescence quenchers were simultaneously used for the assay. The hybridization probes specific for the VARV sequence contained FAM/BHQ1 as a dye/quencher pair; MPXV-specific, JOE/BHQ1; VZV-specific, TAMRA/BHQ2; and internal control-specific, Cy5/BHQ3. The specificity and sensitivity of the developed method were assessed by analyzing DNA of 32 strains belonging to orthopoxvirus and herpesvirus species. Elsevier B.V. 2016-10 2016-07-28 /pmc/articles/PMC9629046/ /pubmed/27477914 http://dx.doi.org/10.1016/j.jviromet.2016.07.024 Text en © 2016 Elsevier B.V. All rights reserved. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Article
Maksyutov, Rinat A.
Gavrilova, Elena V.
Shchelkunov, Sergei N.
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title_full Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title_fullStr Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title_full_unstemmed Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title_short Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay
title_sort species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time pcr assay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629046/
https://www.ncbi.nlm.nih.gov/pubmed/27477914
http://dx.doi.org/10.1016/j.jviromet.2016.07.024
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