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Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity

Abdominal obesity associates with cardiometabolic disease and an accumulation of lipids in the visceral adipose depot, whereas lipid accumulation in the subcutaneous depot is more benign. We aimed to further investigate whether the adipogenic properties where cell-intrinsic, or dependent on a depot-...

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Autores principales: Mathur, Neha, Severinsen, Mai C. K., Jensen, Mette E., Naver, Lars, Schrölkamp, Maren, Laye, Matthew J., Watt, Matthew J., Nielsen, Søren, Krogh-Madsen, Rikke, Pedersen, Bente Klarlund, Scheele, Camilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629396/
https://www.ncbi.nlm.nih.gov/pubmed/36340033
http://dx.doi.org/10.3389/fcell.2022.983899
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author Mathur, Neha
Severinsen, Mai C. K.
Jensen, Mette E.
Naver, Lars
Schrölkamp, Maren
Laye, Matthew J.
Watt, Matthew J.
Nielsen, Søren
Krogh-Madsen, Rikke
Pedersen, Bente Klarlund
Scheele, Camilla
author_facet Mathur, Neha
Severinsen, Mai C. K.
Jensen, Mette E.
Naver, Lars
Schrölkamp, Maren
Laye, Matthew J.
Watt, Matthew J.
Nielsen, Søren
Krogh-Madsen, Rikke
Pedersen, Bente Klarlund
Scheele, Camilla
author_sort Mathur, Neha
collection PubMed
description Abdominal obesity associates with cardiometabolic disease and an accumulation of lipids in the visceral adipose depot, whereas lipid accumulation in the subcutaneous depot is more benign. We aimed to further investigate whether the adipogenic properties where cell-intrinsic, or dependent on a depot-specific or obesity-produced microenvironment. We obtained visceral and subcutaneous biopsies from non-obese women (n = 14) or women living with morbid obesity (n = 14) and isolated adipose stem and progenitor cells (ASPCs) from the stromal vascular fraction of non-obese (n = 13) and obese (n = 13). Following in vitro differentiation into mature adipocytes, we observed a contrasting pattern with a lower gene expression of adipogenic markers and a higher gene expression of immunogenic markers in the visceral compared to the subcutaneous adipocytes. We identified the immunogenic factor BST2 as a marker for visceral ASPCs. The effect of obesity and insulin resistance on adipogenic and immunogenic markers in the in vitro differentiated cells was minor. In contrast, differentiation with exogenous Tumor necrosis factor resulted in increased immunogenic signatures, including increased expression of BST2, and decreased adipogenic signatures in cells from both depots. Our data, from 26 women, underscore the intrinsic differences between human visceral and subcutaneous adipose stem and progenitor cells, suggest that dysregulation of adipocytes in obesity mainly occurs at a post-progenitor stage, and highlight an inflammatory microenvironment as a major constraint of human adipogenesis.
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spelling pubmed-96293962022-11-03 Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity Mathur, Neha Severinsen, Mai C. K. Jensen, Mette E. Naver, Lars Schrölkamp, Maren Laye, Matthew J. Watt, Matthew J. Nielsen, Søren Krogh-Madsen, Rikke Pedersen, Bente Klarlund Scheele, Camilla Front Cell Dev Biol Cell and Developmental Biology Abdominal obesity associates with cardiometabolic disease and an accumulation of lipids in the visceral adipose depot, whereas lipid accumulation in the subcutaneous depot is more benign. We aimed to further investigate whether the adipogenic properties where cell-intrinsic, or dependent on a depot-specific or obesity-produced microenvironment. We obtained visceral and subcutaneous biopsies from non-obese women (n = 14) or women living with morbid obesity (n = 14) and isolated adipose stem and progenitor cells (ASPCs) from the stromal vascular fraction of non-obese (n = 13) and obese (n = 13). Following in vitro differentiation into mature adipocytes, we observed a contrasting pattern with a lower gene expression of adipogenic markers and a higher gene expression of immunogenic markers in the visceral compared to the subcutaneous adipocytes. We identified the immunogenic factor BST2 as a marker for visceral ASPCs. The effect of obesity and insulin resistance on adipogenic and immunogenic markers in the in vitro differentiated cells was minor. In contrast, differentiation with exogenous Tumor necrosis factor resulted in increased immunogenic signatures, including increased expression of BST2, and decreased adipogenic signatures in cells from both depots. Our data, from 26 women, underscore the intrinsic differences between human visceral and subcutaneous adipose stem and progenitor cells, suggest that dysregulation of adipocytes in obesity mainly occurs at a post-progenitor stage, and highlight an inflammatory microenvironment as a major constraint of human adipogenesis. Frontiers Media S.A. 2022-10-17 /pmc/articles/PMC9629396/ /pubmed/36340033 http://dx.doi.org/10.3389/fcell.2022.983899 Text en Copyright © 2022 Mathur, Severinsen, Jensen, Naver, Schrölkamp, Laye, Watt, Nielsen, Krogh-Madsen, Pedersen and Scheele. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Mathur, Neha
Severinsen, Mai C. K.
Jensen, Mette E.
Naver, Lars
Schrölkamp, Maren
Laye, Matthew J.
Watt, Matthew J.
Nielsen, Søren
Krogh-Madsen, Rikke
Pedersen, Bente Klarlund
Scheele, Camilla
Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title_full Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title_fullStr Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title_full_unstemmed Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title_short Human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
title_sort human visceral and subcutaneous adipose stem and progenitor cells retain depot-specific adipogenic properties during obesity
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629396/
https://www.ncbi.nlm.nih.gov/pubmed/36340033
http://dx.doi.org/10.3389/fcell.2022.983899
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