Cargando…

Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae

Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembl...

Descripción completa

Detalles Bibliográficos
Autores principales: Fernandes Santos, Carlos Antonio, Rodrigues da Costa, Soniane, Silva Boiteux, Leonardo, Grattapaglia, Dario, Silva-Junior, Orzenil Bonfim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629644/
https://www.ncbi.nlm.nih.gov/pubmed/36322533
http://dx.doi.org/10.1371/journal.pone.0273959
_version_ 1784823442639421440
author Fernandes Santos, Carlos Antonio
Rodrigues da Costa, Soniane
Silva Boiteux, Leonardo
Grattapaglia, Dario
Silva-Junior, Orzenil Bonfim
author_facet Fernandes Santos, Carlos Antonio
Rodrigues da Costa, Soniane
Silva Boiteux, Leonardo
Grattapaglia, Dario
Silva-Junior, Orzenil Bonfim
author_sort Fernandes Santos, Carlos Antonio
collection PubMed
description Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleotide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloidogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks.
format Online
Article
Text
id pubmed-9629644
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-96296442022-11-03 Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae Fernandes Santos, Carlos Antonio Rodrigues da Costa, Soniane Silva Boiteux, Leonardo Grattapaglia, Dario Silva-Junior, Orzenil Bonfim PLoS One Research Article Tropical fruit tree species constitute a yet untapped supply of outstanding diversity of taste and nutritional value, barely developed from the genetics standpoint, with scarce or no genomic resources to tackle the challenges arising in modern breeding practice. We generated a de novo genome assembly of the Psidium guajava, the super fruit “apple of the tropics”, and successfully transferred 14,268 SNP probesets from Eucalyptus to Psidium at the nucleotide level, to detect genomic loci linked to resistance to the root knot nematode (RKN) Meloidogyne enterolobii derived from the wild relative P. guineense. Significantly associated loci with resistance across alternative analytical frameworks, were detected at two SNPs on chromosome 3 in a pseudo-assembly of Psidium guajava genome built using a syntenic path approach with the Eucalyptus grandis genome to determine the order and orientation of the contigs. The P. guineense-derived resistance response to RKN and disease onset is conceivably triggered by mineral nutrients and phytohormone homeostasis or signaling with the involvement of the miRNA pathway. Hotspots of mapped resistance quantitative trait loci and functional annotation in the same genomic region of Eucalyptus provide further indirect support to our results, highlighting the evolutionary conservation of genomes across genera of Myrtaceae in the adaptation to pathogens. Marker assisted introgression of the resistance loci mapped should accelerate the development of improved guava cultivars and hybrid rootstocks. Public Library of Science 2022-11-02 /pmc/articles/PMC9629644/ /pubmed/36322533 http://dx.doi.org/10.1371/journal.pone.0273959 Text en © 2022 Fernandes Santos et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fernandes Santos, Carlos Antonio
Rodrigues da Costa, Soniane
Silva Boiteux, Leonardo
Grattapaglia, Dario
Silva-Junior, Orzenil Bonfim
Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title_full Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title_fullStr Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title_full_unstemmed Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title_short Genetic associations with resistance to Meloidogyne enterolobii in guava (Psidium sp.) using cross-genera SNPs and comparative genomics to Eucalyptus highlight evolutionary conservation across the Myrtaceae
title_sort genetic associations with resistance to meloidogyne enterolobii in guava (psidium sp.) using cross-genera snps and comparative genomics to eucalyptus highlight evolutionary conservation across the myrtaceae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629644/
https://www.ncbi.nlm.nih.gov/pubmed/36322533
http://dx.doi.org/10.1371/journal.pone.0273959
work_keys_str_mv AT fernandessantoscarlosantonio geneticassociationswithresistancetomeloidogyneenterolobiiinguavapsidiumspusingcrossgenerasnpsandcomparativegenomicstoeucalyptushighlightevolutionaryconservationacrossthemyrtaceae
AT rodriguesdacostasoniane geneticassociationswithresistancetomeloidogyneenterolobiiinguavapsidiumspusingcrossgenerasnpsandcomparativegenomicstoeucalyptushighlightevolutionaryconservationacrossthemyrtaceae
AT silvaboiteuxleonardo geneticassociationswithresistancetomeloidogyneenterolobiiinguavapsidiumspusingcrossgenerasnpsandcomparativegenomicstoeucalyptushighlightevolutionaryconservationacrossthemyrtaceae
AT grattapagliadario geneticassociationswithresistancetomeloidogyneenterolobiiinguavapsidiumspusingcrossgenerasnpsandcomparativegenomicstoeucalyptushighlightevolutionaryconservationacrossthemyrtaceae
AT silvajuniororzenilbonfim geneticassociationswithresistancetomeloidogyneenterolobiiinguavapsidiumspusingcrossgenerasnpsandcomparativegenomicstoeucalyptushighlightevolutionaryconservationacrossthemyrtaceae