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Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant
BACKGROUND: The SARS-CoV-2 variant B.1.1.529 potentially escapes immunity from vaccination via a heavily mutated Spike protein. Here, we analyzed whether T cell memory towards the B.1.1.529 Spike protein is present in individuals who received two or three doses of vaccines designed against the origi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629766/ https://www.ncbi.nlm.nih.gov/pubmed/36352067 http://dx.doi.org/10.1038/s43856-022-00203-7 |
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author | Dörnte, Charlyn Traska, Verena Jansen, Nicole Kostyra, Julia Baurmann, Herrad Lauer, Gereon Huang, Yi-Ju Kramer, Sven Brauns, Olaf Winkels, Holger Schmitz, Jürgen Dose, Christian Richter, Anne Schuster, Marc |
author_facet | Dörnte, Charlyn Traska, Verena Jansen, Nicole Kostyra, Julia Baurmann, Herrad Lauer, Gereon Huang, Yi-Ju Kramer, Sven Brauns, Olaf Winkels, Holger Schmitz, Jürgen Dose, Christian Richter, Anne Schuster, Marc |
author_sort | Dörnte, Charlyn |
collection | PubMed |
description | BACKGROUND: The SARS-CoV-2 variant B.1.1.529 potentially escapes immunity from vaccination via a heavily mutated Spike protein. Here, we analyzed whether T cell memory towards the B.1.1.529 Spike protein is present in individuals who received two or three doses of vaccines designed against the original Wuhan strain of SARS-CoV-2. METHODS: PBMCs were isolated from two- and three-times vaccinated study participants and incubated in vitro with peptide pools of the Spike protein derived from sequences of the original Wuhan or the B.1.1.529 strains of SARS-CoV-2. Activated antigen-specific T cells were detected by flow cytometry. In silico analyses with NetMHCpan and NetMHCIIpan were used to determine differences in MHC class presentation between the original strain and the B.1.1.529 strain for the most common MHCs in the European-Caucasian population. RESULTS: Here we show, that both CD4 and CD8 responses to the B.1.1.529 Spike protein are marginally reduced compared to the ancestor protein and a robust T cell response is maintained. Epitope analyses reveal minor differences between the two SARS-CoV-2 strains in terms of MHC class presentations for the MHC-alleles being most common in the European-Caucasian population. CONCLUSIONS: The memory T cell response induced via first generation vaccination remains robust and is mostly unaffected by B.1.1.529 mutations. Correspondingly, in silico analyses of MHC presentation of epitopes derived from the B.1.1.529 Spike protein shows marginal differences compared to the ancestral SARS-CoV-2 strain. |
format | Online Article Text |
id | pubmed-9629766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96297662022-11-03 Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant Dörnte, Charlyn Traska, Verena Jansen, Nicole Kostyra, Julia Baurmann, Herrad Lauer, Gereon Huang, Yi-Ju Kramer, Sven Brauns, Olaf Winkels, Holger Schmitz, Jürgen Dose, Christian Richter, Anne Schuster, Marc Commun Med (Lond) Article BACKGROUND: The SARS-CoV-2 variant B.1.1.529 potentially escapes immunity from vaccination via a heavily mutated Spike protein. Here, we analyzed whether T cell memory towards the B.1.1.529 Spike protein is present in individuals who received two or three doses of vaccines designed against the original Wuhan strain of SARS-CoV-2. METHODS: PBMCs were isolated from two- and three-times vaccinated study participants and incubated in vitro with peptide pools of the Spike protein derived from sequences of the original Wuhan or the B.1.1.529 strains of SARS-CoV-2. Activated antigen-specific T cells were detected by flow cytometry. In silico analyses with NetMHCpan and NetMHCIIpan were used to determine differences in MHC class presentation between the original strain and the B.1.1.529 strain for the most common MHCs in the European-Caucasian population. RESULTS: Here we show, that both CD4 and CD8 responses to the B.1.1.529 Spike protein are marginally reduced compared to the ancestor protein and a robust T cell response is maintained. Epitope analyses reveal minor differences between the two SARS-CoV-2 strains in terms of MHC class presentations for the MHC-alleles being most common in the European-Caucasian population. CONCLUSIONS: The memory T cell response induced via first generation vaccination remains robust and is mostly unaffected by B.1.1.529 mutations. Correspondingly, in silico analyses of MHC presentation of epitopes derived from the B.1.1.529 Spike protein shows marginal differences compared to the ancestral SARS-CoV-2 strain. Nature Publishing Group UK 2022-11-02 /pmc/articles/PMC9629766/ /pubmed/36352067 http://dx.doi.org/10.1038/s43856-022-00203-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Dörnte, Charlyn Traska, Verena Jansen, Nicole Kostyra, Julia Baurmann, Herrad Lauer, Gereon Huang, Yi-Ju Kramer, Sven Brauns, Olaf Winkels, Holger Schmitz, Jürgen Dose, Christian Richter, Anne Schuster, Marc Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title | Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title_full | Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title_fullStr | Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title_full_unstemmed | Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title_short | Vaccines against the original strain of SARS-CoV-2 provide T cell memory to the B.1.1.529 variant |
title_sort | vaccines against the original strain of sars-cov-2 provide t cell memory to the b.1.1.529 variant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629766/ https://www.ncbi.nlm.nih.gov/pubmed/36352067 http://dx.doi.org/10.1038/s43856-022-00203-7 |
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