Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)

Pulmonary atresia with intact ventricular septum (PA-IVS) is a rare congenital heart defect defined by membranous or muscular atresia of the right ventricular outflow tract where patients display varying degrees of hypoplasia of the right ventricle. This condition results in cyanosis due to an inabi...

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Autores principales: Contreras, Javier, Alonzo, Matthew, Ye, Shiqiao, Lin, Hui, Hernandez-Rosario, Lumariz, McBride, Kim L., Texter, Karen, Garg, Vidu, Zhao, Ming-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629876/
https://www.ncbi.nlm.nih.gov/pubmed/35987120
http://dx.doi.org/10.1016/j.scr.2022.102893
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author Contreras, Javier
Alonzo, Matthew
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
author_facet Contreras, Javier
Alonzo, Matthew
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
author_sort Contreras, Javier
collection PubMed
description Pulmonary atresia with intact ventricular septum (PA-IVS) is a rare congenital heart defect defined by membranous or muscular atresia of the right ventricular outflow tract where patients display varying degrees of hypoplasia of the right ventricle. This condition results in cyanosis due to an inability of blood to flow from the right ventricle to the pulmonary arteries, thus requiring immediate surgical intervention after birth. An iPSC line was generated from peripheral blood mononuclear cells of a 11-year-old male patient diagnosed with PA-IVS through Sendai virus-mediated reprogramming. This disease-specific iPSC line was characterized by immunocytochemistry, STR analysis, karyotype analysis, and mycoplasma testing.
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spelling pubmed-96298762022-11-02 Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS) Contreras, Javier Alonzo, Matthew Ye, Shiqiao Lin, Hui Hernandez-Rosario, Lumariz McBride, Kim L. Texter, Karen Garg, Vidu Zhao, Ming-Tao Stem Cell Res Article Pulmonary atresia with intact ventricular septum (PA-IVS) is a rare congenital heart defect defined by membranous or muscular atresia of the right ventricular outflow tract where patients display varying degrees of hypoplasia of the right ventricle. This condition results in cyanosis due to an inability of blood to flow from the right ventricle to the pulmonary arteries, thus requiring immediate surgical intervention after birth. An iPSC line was generated from peripheral blood mononuclear cells of a 11-year-old male patient diagnosed with PA-IVS through Sendai virus-mediated reprogramming. This disease-specific iPSC line was characterized by immunocytochemistry, STR analysis, karyotype analysis, and mycoplasma testing. 2022-10 2022-08-10 /pmc/articles/PMC9629876/ /pubmed/35987120 http://dx.doi.org/10.1016/j.scr.2022.102893 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Contreras, Javier
Alonzo, Matthew
Ye, Shiqiao
Lin, Hui
Hernandez-Rosario, Lumariz
McBride, Kim L.
Texter, Karen
Garg, Vidu
Zhao, Ming-Tao
Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title_full Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title_fullStr Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title_full_unstemmed Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title_short Generation of an induced pluripotent stem cell line NCHi003-A from a 11-year-old male with pulmonary atresia with intact ventricular septum (PA-IVS)
title_sort generation of an induced pluripotent stem cell line nchi003-a from a 11-year-old male with pulmonary atresia with intact ventricular septum (pa-ivs)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629876/
https://www.ncbi.nlm.nih.gov/pubmed/35987120
http://dx.doi.org/10.1016/j.scr.2022.102893
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