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Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus

To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregna...

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Autores principales: Ye, Zhixin, Wang, Songzi, Huang, Xiaoqing, Chen, Peisong, Deng, Langhui, Li, Shiqi, Lin, Suiwen, Wang, Zilian, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630082/
https://www.ncbi.nlm.nih.gov/pubmed/35926094
http://dx.doi.org/10.2337/db21-0909
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author Ye, Zhixin
Wang, Songzi
Huang, Xiaoqing
Chen, Peisong
Deng, Langhui
Li, Shiqi
Lin, Suiwen
Wang, Zilian
Liu, Bin
author_facet Ye, Zhixin
Wang, Songzi
Huang, Xiaoqing
Chen, Peisong
Deng, Langhui
Li, Shiqi
Lin, Suiwen
Wang, Zilian
Liu, Bin
author_sort Ye, Zhixin
collection PubMed
description To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423–5p was upregulated, whereas miR-122–5p, miR-148a-3p, miR-192–5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423–5p, and G6PC3 and FDFT1 as target genes of miR-122–5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM.
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spelling pubmed-96300822023-01-21 Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus Ye, Zhixin Wang, Songzi Huang, Xiaoqing Chen, Peisong Deng, Langhui Li, Shiqi Lin, Suiwen Wang, Zilian Liu, Bin Diabetes Metabolism To date, the miRNA expression profile of plasma exosomes in women whose pregnancy is complicated by gestational diabetes mellitus (GDM) has not been fully clarified. In this study, differentially expressed miRNAs in plasma exosomes were identified by high-throughput small-RNA sequencing in 12 pregnant women with GDM and 12 with normal glucose tolerance (NGT) and validated in 102 pregnant women with GDM and 101 with NGT. A total of 22 exosomal miRNAs were found, five of which were verified by real-time qPCR. Exosomal miR-423–5p was upregulated, whereas miR-122–5p, miR-148a-3p, miR-192–5p, and miR-99a-5p were downregulated in women whose pregnancy was complicated by GDM. IGF1R and GYS1 as target genes of miR-423–5p, and G6PC3 and FDFT1 as target genes of miR-122–5p were associated with insulin and AMPK signaling pathways and may participate in the regulation of metabolism in GDM. The five exosomal miRNAs had an area under the curve of 0.82 (95%CI, 0.73, ∼0.91) in early prediction of GDM. Our study demonstrates that dysregulated exosomal miRNAs in plasma from pregnant women with GDM might influence the insulin and AMPK signaling pathways and could contribute to the early prediction of GDM. American Diabetes Association 2022-11 2022-08-04 /pmc/articles/PMC9630082/ /pubmed/35926094 http://dx.doi.org/10.2337/db21-0909 Text en © 2022 by the American Diabetes Association https://www.diabetesjournals.org/journals/pages/licenseReaders may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at https://www.diabetesjournals.org/journals/pages/license.
spellingShingle Metabolism
Ye, Zhixin
Wang, Songzi
Huang, Xiaoqing
Chen, Peisong
Deng, Langhui
Li, Shiqi
Lin, Suiwen
Wang, Zilian
Liu, Bin
Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title_full Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title_fullStr Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title_full_unstemmed Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title_short Plasma Exosomal miRNAs Associated With Metabolism as Early Predictor of Gestational Diabetes Mellitus
title_sort plasma exosomal mirnas associated with metabolism as early predictor of gestational diabetes mellitus
topic Metabolism
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630082/
https://www.ncbi.nlm.nih.gov/pubmed/35926094
http://dx.doi.org/10.2337/db21-0909
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