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Fourier analysis of collagen bundle orientation in myocardial infarction scars
Collagen bundle orientation (CBO) in myocardial infarct scars plays a major role in scar mechanics and complications after infarction. We aim to compare four histopathological methods for CBO measurement in myocardial scarring. Myocardial infarction was induced in 21 pigs by balloon coronary occlusi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630212/ https://www.ncbi.nlm.nih.gov/pubmed/35948735 http://dx.doi.org/10.1007/s00418-022-02132-x |
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author | Marcos-Garcés, Víctor Rios-Navarro, Cesar Gómez-Torres, Fabián Gavara, Jose de Dios, Elena Diaz, Ana Miñana, Gema Chorro, Francisco Javier Bodi, Vicente Ruiz-Sauri, Amparo |
author_facet | Marcos-Garcés, Víctor Rios-Navarro, Cesar Gómez-Torres, Fabián Gavara, Jose de Dios, Elena Diaz, Ana Miñana, Gema Chorro, Francisco Javier Bodi, Vicente Ruiz-Sauri, Amparo |
author_sort | Marcos-Garcés, Víctor |
collection | PubMed |
description | Collagen bundle orientation (CBO) in myocardial infarct scars plays a major role in scar mechanics and complications after infarction. We aim to compare four histopathological methods for CBO measurement in myocardial scarring. Myocardial infarction was induced in 21 pigs by balloon coronary occlusion. Scar samples were obtained at 4 weeks, stained with Masson’s trichrome, Picrosirius red, and Hematoxylin–Eosin (H&E), and photographed using light, polarized light microscopy, and confocal microscopy, respectively. Masson’s trichrome images were also optimized to remove non-collagenous structures. Two observers measured CBO by means of a semi-automated, Fourier analysis protocol. Interrater reliability and comparability between techniques were studied by the intraclass correlation coefficient (ICC) and Bland–Altman (B&A) plots and limits of agreement. Fourier analysis showed an almost perfect interrater reliability for each technique (ICC ≥ 0.95, p < 0.001 in all cases). CBO showed more randomly oriented values in Masson’s trichrome and worse comparability with other techniques (ICC vs. Picrosirius red: 0.79 [0.47–0.91], p = 0.001; vs. H&E-confocal: 0.70 [0.26–0.88], p = 0.005). However, optimized Masson’s trichrome showed almost perfect agreement with Picrosirius red (ICC 0.84 [0.6–0.94], p < 0.001) and H&E-confocal (ICC 0.81 [0.54–0.92], p < 0.001), as well as these latter techniques between each other (ICC 0.84 [0.60–0.93], p < 0.001). In summary, a semi-automated, Fourier-based method can provide highly reproducible CBO measurements in four different histopathological techniques. Masson’s trichrome tends to provide more randomly oriented CBO index values, probably due to non-specific visualization of non-collagenous structures. However, optimization of Masson’s trichrome microphotographs to remove non-collagenous components provides an almost perfect comparability between this technique, Picrosirius red and H&E-confocal. |
format | Online Article Text |
id | pubmed-9630212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-96302122022-11-04 Fourier analysis of collagen bundle orientation in myocardial infarction scars Marcos-Garcés, Víctor Rios-Navarro, Cesar Gómez-Torres, Fabián Gavara, Jose de Dios, Elena Diaz, Ana Miñana, Gema Chorro, Francisco Javier Bodi, Vicente Ruiz-Sauri, Amparo Histochem Cell Biol Original Paper Collagen bundle orientation (CBO) in myocardial infarct scars plays a major role in scar mechanics and complications after infarction. We aim to compare four histopathological methods for CBO measurement in myocardial scarring. Myocardial infarction was induced in 21 pigs by balloon coronary occlusion. Scar samples were obtained at 4 weeks, stained with Masson’s trichrome, Picrosirius red, and Hematoxylin–Eosin (H&E), and photographed using light, polarized light microscopy, and confocal microscopy, respectively. Masson’s trichrome images were also optimized to remove non-collagenous structures. Two observers measured CBO by means of a semi-automated, Fourier analysis protocol. Interrater reliability and comparability between techniques were studied by the intraclass correlation coefficient (ICC) and Bland–Altman (B&A) plots and limits of agreement. Fourier analysis showed an almost perfect interrater reliability for each technique (ICC ≥ 0.95, p < 0.001 in all cases). CBO showed more randomly oriented values in Masson’s trichrome and worse comparability with other techniques (ICC vs. Picrosirius red: 0.79 [0.47–0.91], p = 0.001; vs. H&E-confocal: 0.70 [0.26–0.88], p = 0.005). However, optimized Masson’s trichrome showed almost perfect agreement with Picrosirius red (ICC 0.84 [0.6–0.94], p < 0.001) and H&E-confocal (ICC 0.81 [0.54–0.92], p < 0.001), as well as these latter techniques between each other (ICC 0.84 [0.60–0.93], p < 0.001). In summary, a semi-automated, Fourier-based method can provide highly reproducible CBO measurements in four different histopathological techniques. Masson’s trichrome tends to provide more randomly oriented CBO index values, probably due to non-specific visualization of non-collagenous structures. However, optimization of Masson’s trichrome microphotographs to remove non-collagenous components provides an almost perfect comparability between this technique, Picrosirius red and H&E-confocal. Springer Berlin Heidelberg 2022-08-10 2022 /pmc/articles/PMC9630212/ /pubmed/35948735 http://dx.doi.org/10.1007/s00418-022-02132-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Paper Marcos-Garcés, Víctor Rios-Navarro, Cesar Gómez-Torres, Fabián Gavara, Jose de Dios, Elena Diaz, Ana Miñana, Gema Chorro, Francisco Javier Bodi, Vicente Ruiz-Sauri, Amparo Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title | Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title_full | Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title_fullStr | Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title_full_unstemmed | Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title_short | Fourier analysis of collagen bundle orientation in myocardial infarction scars |
title_sort | fourier analysis of collagen bundle orientation in myocardial infarction scars |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630212/ https://www.ncbi.nlm.nih.gov/pubmed/35948735 http://dx.doi.org/10.1007/s00418-022-02132-x |
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