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Impaired intratumoral natural killer cell function in head and neck carcinoma
Natural killer (NK) cells are emerging as unique players in the immune response against cancer; however, only limited data are available on tumor infiltrating NK cells in head and neck squamous cell carcinoma (HNSCC), one of the most common cancer. Occurrence of HNSCC is closely related to the immun...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630640/ https://www.ncbi.nlm.nih.gov/pubmed/36341402 http://dx.doi.org/10.3389/fimmu.2022.997806 |
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author | Mele, Dalila Pessino, Greta Trisolini, Giuseppe Luchena, Alberto Benazzo, Marco Morbini, Patrizia Mantovani, Stefania Oliviero, Barbara Mondelli, Mario U. Varchetta, Stefania |
author_facet | Mele, Dalila Pessino, Greta Trisolini, Giuseppe Luchena, Alberto Benazzo, Marco Morbini, Patrizia Mantovani, Stefania Oliviero, Barbara Mondelli, Mario U. Varchetta, Stefania |
author_sort | Mele, Dalila |
collection | PubMed |
description | Natural killer (NK) cells are emerging as unique players in the immune response against cancer; however, only limited data are available on tumor infiltrating NK cells in head and neck squamous cell carcinoma (HNSCC), one of the most common cancer. Occurrence of HNSCC is closely related to the immune microenvironment, and immunotherapy is increasingly being applied to this setting. However, the limited success of this type of treatment in this tumor calls for further investigation in the field. Surgical HNSSC specimens of 32 consecutive patients were mechanically and enzymatically dissociated. Tumor cells were separated from infiltrating cells by short centrifugation and infiltrating NK cells were phenotypically and functionally characterized by multiple antibody staining and flow cytometry. Tumor infiltrating NK cells in HNSCC showed a peculiar phenotype predominantly characterized by increased NKG2A and reduced Siglec-7, NKG2D, NKp30 and CD16 expression. This phenotype was associated with a decreased ability to perform antibody-dependent cellular cytotoxicity (ADCC). However, NK, CD4 and CD8 shared an increment of glucocorticoid-induced tumor necrosis factor-related (GITR) costimulatory receptor which could be exploited for immunotherapy with agonistic anti-GITR antibodies combined with checkpoint inhibitors. |
format | Online Article Text |
id | pubmed-9630640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96306402022-11-04 Impaired intratumoral natural killer cell function in head and neck carcinoma Mele, Dalila Pessino, Greta Trisolini, Giuseppe Luchena, Alberto Benazzo, Marco Morbini, Patrizia Mantovani, Stefania Oliviero, Barbara Mondelli, Mario U. Varchetta, Stefania Front Immunol Immunology Natural killer (NK) cells are emerging as unique players in the immune response against cancer; however, only limited data are available on tumor infiltrating NK cells in head and neck squamous cell carcinoma (HNSCC), one of the most common cancer. Occurrence of HNSCC is closely related to the immune microenvironment, and immunotherapy is increasingly being applied to this setting. However, the limited success of this type of treatment in this tumor calls for further investigation in the field. Surgical HNSSC specimens of 32 consecutive patients were mechanically and enzymatically dissociated. Tumor cells were separated from infiltrating cells by short centrifugation and infiltrating NK cells were phenotypically and functionally characterized by multiple antibody staining and flow cytometry. Tumor infiltrating NK cells in HNSCC showed a peculiar phenotype predominantly characterized by increased NKG2A and reduced Siglec-7, NKG2D, NKp30 and CD16 expression. This phenotype was associated with a decreased ability to perform antibody-dependent cellular cytotoxicity (ADCC). However, NK, CD4 and CD8 shared an increment of glucocorticoid-induced tumor necrosis factor-related (GITR) costimulatory receptor which could be exploited for immunotherapy with agonistic anti-GITR antibodies combined with checkpoint inhibitors. Frontiers Media S.A. 2022-10-20 /pmc/articles/PMC9630640/ /pubmed/36341402 http://dx.doi.org/10.3389/fimmu.2022.997806 Text en Copyright © 2022 Mele, Pessino, Trisolini, Luchena, Benazzo, Morbini, Mantovani, Oliviero, Mondelli and Varchetta https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Mele, Dalila Pessino, Greta Trisolini, Giuseppe Luchena, Alberto Benazzo, Marco Morbini, Patrizia Mantovani, Stefania Oliviero, Barbara Mondelli, Mario U. Varchetta, Stefania Impaired intratumoral natural killer cell function in head and neck carcinoma |
title | Impaired intratumoral natural killer cell function in head and neck carcinoma |
title_full | Impaired intratumoral natural killer cell function in head and neck carcinoma |
title_fullStr | Impaired intratumoral natural killer cell function in head and neck carcinoma |
title_full_unstemmed | Impaired intratumoral natural killer cell function in head and neck carcinoma |
title_short | Impaired intratumoral natural killer cell function in head and neck carcinoma |
title_sort | impaired intratumoral natural killer cell function in head and neck carcinoma |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630640/ https://www.ncbi.nlm.nih.gov/pubmed/36341402 http://dx.doi.org/10.3389/fimmu.2022.997806 |
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