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Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates

Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral...

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Detalles Bibliográficos
Autores principales: Sun, Difang, Wang, Bin, Yang, Zhenlan, Zhan, Zongyi, Li, Jun, Lan, Yuqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630776/
https://www.ncbi.nlm.nih.gov/pubmed/36340883
http://dx.doi.org/10.1016/j.xpro.2022.101801
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author Sun, Difang
Wang, Bin
Yang, Zhenlan
Zhan, Zongyi
Li, Jun
Lan, Yuqing
author_facet Sun, Difang
Wang, Bin
Yang, Zhenlan
Zhan, Zongyi
Li, Jun
Lan, Yuqing
author_sort Sun, Difang
collection PubMed
description Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed. For complete details on the use and execution of this protocol, please refer to Sun et al. (2022).
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spelling pubmed-96307762022-11-04 Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates Sun, Difang Wang, Bin Yang, Zhenlan Zhan, Zongyi Li, Jun Lan, Yuqing STAR Protoc Protocol Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed. For complete details on the use and execution of this protocol, please refer to Sun et al. (2022). Elsevier 2022-10-29 /pmc/articles/PMC9630776/ /pubmed/36340883 http://dx.doi.org/10.1016/j.xpro.2022.101801 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sun, Difang
Wang, Bin
Yang, Zhenlan
Zhan, Zongyi
Li, Jun
Lan, Yuqing
Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title_full Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title_fullStr Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title_full_unstemmed Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title_short Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
title_sort protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630776/
https://www.ncbi.nlm.nih.gov/pubmed/36340883
http://dx.doi.org/10.1016/j.xpro.2022.101801
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