Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods

BACKGROUND AND AIM: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/Cas9) system. Different delivery methods, such as microinjectio...

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Detalles Bibliográficos
Autores principales: Le, Quynh Anh, Wittayarat, Manita, Namula, Zhao, Lin, Qingyi, Takebayashi, Koki, Hirata, Maki, Tanihara, Fuminori, Do, Lanh Thi Kim, Otoi, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631378/
https://www.ncbi.nlm.nih.gov/pubmed/36341066
http://dx.doi.org/10.14202/vetworld.2022.2210-2216

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631378/
https://www.ncbi.nlm.nih.gov/pubmed/36341066
http://dx.doi.org/10.14202/vetworld.2022.2210-2216

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