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Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods
BACKGROUND AND AIM: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/Cas9) system. Different delivery methods, such as microinjectio...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Veterinary World
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631378/ https://www.ncbi.nlm.nih.gov/pubmed/36341066 http://dx.doi.org/10.14202/vetworld.2022.2210-2216 |