Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses

A 14-kDa gene-specific probe of vaccinia virus Western Reserve (WR) hybridized to homologous sequences in the genomes of the orthopox virus species cowpox, camelpox, mousepox, and monkeypox virus. The corresponding genes were mapped and sequenced. Homologies of more than 95% were found when compared...

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Detalles Bibliográficos
Autores principales: Meyer, Hermann, Osterrieder, Nikolaus, Czerny, Claus-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press. 1994
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631423/
https://www.ncbi.nlm.nih.gov/pubmed/7513923
http://dx.doi.org/10.1006/viro.1994.1241
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author Meyer, Hermann
Osterrieder, Nikolaus
Czerny, Claus-Peter
author_facet Meyer, Hermann
Osterrieder, Nikolaus
Czerny, Claus-Peter
author_sort Meyer, Hermann
collection PubMed
description A 14-kDa gene-specific probe of vaccinia virus Western Reserve (WR) hybridized to homologous sequences in the genomes of the orthopox virus species cowpox, camelpox, mousepox, and monkeypox virus. The corresponding genes were mapped and sequenced. Homologies of more than 95% were found when compared to the 14-kDa gene of vaccinia virus WR. However, point mutations which led to alterations in the amino acid sequences were mainly located between residues 26 and 40. By use of synthetic peptides, this part of the 14-kDa fusion protein could be identified as the binding site for four different neutralizing monoclonal antibodies.
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spelling pubmed-96314232022-11-03 Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses Meyer, Hermann Osterrieder, Nikolaus Czerny, Claus-Peter Virology Short Communications A 14-kDa gene-specific probe of vaccinia virus Western Reserve (WR) hybridized to homologous sequences in the genomes of the orthopox virus species cowpox, camelpox, mousepox, and monkeypox virus. The corresponding genes were mapped and sequenced. Homologies of more than 95% were found when compared to the 14-kDa gene of vaccinia virus WR. However, point mutations which led to alterations in the amino acid sequences were mainly located between residues 26 and 40. By use of synthetic peptides, this part of the 14-kDa fusion protein could be identified as the binding site for four different neutralizing monoclonal antibodies. Academic Press. 1994-05-01 2002-05-25 /pmc/articles/PMC9631423/ /pubmed/7513923 http://dx.doi.org/10.1006/viro.1994.1241 Text en Copyright © 1994 Academic Press. All rights reserved. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Short Communications
Meyer, Hermann
Osterrieder, Nikolaus
Czerny, Claus-Peter
Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title_full Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title_fullStr Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title_full_unstemmed Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title_short Identification of Binding Sites for Neutralizing Monoclonal Antibodies on the 14-kDa Fusion Protein of Orthopox Viruses
title_sort identification of binding sites for neutralizing monoclonal antibodies on the 14-kda fusion protein of orthopox viruses
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631423/
https://www.ncbi.nlm.nih.gov/pubmed/7513923
http://dx.doi.org/10.1006/viro.1994.1241
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