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Exploratory studies of oral and fecal microbiome in healthy human aging

Growing evidence has linked an altered host fecal microbiome composition with health status, common chronic diseases, and institutionalization in vulnerable older adults. However, fewer studies have described microbiome changes in healthy older adults without major confounding diseases or conditions...

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Autores principales: Zhou, Xin, Wang, Baohong, Demkowicz, Patrick C., Johnson, Jethro S., Chen, Yanfei, Spakowicz, Daniel J., Zhou, Yanjiao, Dorsett, Yair, Chen, Lei, Sodergren, Erica, Kuchel, George A., Weinstock, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631447/
https://www.ncbi.nlm.nih.gov/pubmed/36338834
http://dx.doi.org/10.3389/fragi.2022.1002405
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author Zhou, Xin
Wang, Baohong
Demkowicz, Patrick C.
Johnson, Jethro S.
Chen, Yanfei
Spakowicz, Daniel J.
Zhou, Yanjiao
Dorsett, Yair
Chen, Lei
Sodergren, Erica
Kuchel, George A.
Weinstock, George M.
author_facet Zhou, Xin
Wang, Baohong
Demkowicz, Patrick C.
Johnson, Jethro S.
Chen, Yanfei
Spakowicz, Daniel J.
Zhou, Yanjiao
Dorsett, Yair
Chen, Lei
Sodergren, Erica
Kuchel, George A.
Weinstock, George M.
author_sort Zhou, Xin
collection PubMed
description Growing evidence has linked an altered host fecal microbiome composition with health status, common chronic diseases, and institutionalization in vulnerable older adults. However, fewer studies have described microbiome changes in healthy older adults without major confounding diseases or conditions, and the impact of aging on the microbiome across different body sites remains unknown. Using 16S ribosomal RNA gene sequencing, we reconstructed the composition of oral and fecal microbiomes in young (23–32; mean = 25 years old) and older (69–94; mean = 77 years old) healthy community-dwelling research subjects. In both body sites, we identified changes in minor bacterial operational taxonomic units (OTUs) between young and older subjects. However, the composition of the predominant bacterial species of the healthy older group in both microbiomes was not significantly different from that of the young cohort, which suggests that dominant bacterial species are relatively stable with healthy aging. In addition, the relative abundance of potentially pathogenic genera, such as Rothia and Mycoplasma, was enriched in the oral microbiome of the healthy older group relative to the young cohort. We also identified several OTUs with a prevalence above 40% and some were more common in young and others in healthy older adults. Differences with aging varied for oral and fecal samples, which suggests that members of the microbiome may be differentially affected by aging in a tissue-specific fashion. This is the first study to investigate both oral and fecal microbiomes in the context of human aging, and provides new insights into interactions between aging and the microbiome within two different clinically relevant sites.
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spelling pubmed-96314472022-11-04 Exploratory studies of oral and fecal microbiome in healthy human aging Zhou, Xin Wang, Baohong Demkowicz, Patrick C. Johnson, Jethro S. Chen, Yanfei Spakowicz, Daniel J. Zhou, Yanjiao Dorsett, Yair Chen, Lei Sodergren, Erica Kuchel, George A. Weinstock, George M. Front Aging Aging Growing evidence has linked an altered host fecal microbiome composition with health status, common chronic diseases, and institutionalization in vulnerable older adults. However, fewer studies have described microbiome changes in healthy older adults without major confounding diseases or conditions, and the impact of aging on the microbiome across different body sites remains unknown. Using 16S ribosomal RNA gene sequencing, we reconstructed the composition of oral and fecal microbiomes in young (23–32; mean = 25 years old) and older (69–94; mean = 77 years old) healthy community-dwelling research subjects. In both body sites, we identified changes in minor bacterial operational taxonomic units (OTUs) between young and older subjects. However, the composition of the predominant bacterial species of the healthy older group in both microbiomes was not significantly different from that of the young cohort, which suggests that dominant bacterial species are relatively stable with healthy aging. In addition, the relative abundance of potentially pathogenic genera, such as Rothia and Mycoplasma, was enriched in the oral microbiome of the healthy older group relative to the young cohort. We also identified several OTUs with a prevalence above 40% and some were more common in young and others in healthy older adults. Differences with aging varied for oral and fecal samples, which suggests that members of the microbiome may be differentially affected by aging in a tissue-specific fashion. This is the first study to investigate both oral and fecal microbiomes in the context of human aging, and provides new insights into interactions between aging and the microbiome within two different clinically relevant sites. Frontiers Media S.A. 2022-10-20 /pmc/articles/PMC9631447/ /pubmed/36338834 http://dx.doi.org/10.3389/fragi.2022.1002405 Text en Copyright © 2022 Zhou, Wang, Demkowicz, Johnson, Chen, Spakowicz, Zhou, Dorsett, Chen, Sodergren, Kuchel and Weinstock. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging
Zhou, Xin
Wang, Baohong
Demkowicz, Patrick C.
Johnson, Jethro S.
Chen, Yanfei
Spakowicz, Daniel J.
Zhou, Yanjiao
Dorsett, Yair
Chen, Lei
Sodergren, Erica
Kuchel, George A.
Weinstock, George M.
Exploratory studies of oral and fecal microbiome in healthy human aging
title Exploratory studies of oral and fecal microbiome in healthy human aging
title_full Exploratory studies of oral and fecal microbiome in healthy human aging
title_fullStr Exploratory studies of oral and fecal microbiome in healthy human aging
title_full_unstemmed Exploratory studies of oral and fecal microbiome in healthy human aging
title_short Exploratory studies of oral and fecal microbiome in healthy human aging
title_sort exploratory studies of oral and fecal microbiome in healthy human aging
topic Aging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631447/
https://www.ncbi.nlm.nih.gov/pubmed/36338834
http://dx.doi.org/10.3389/fragi.2022.1002405
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