Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()

A combinatorial, phage-display library of human Fab antibody fragments was generated from IgG heavy chain (HC) and light chain (LC) genes cloned from the lymphocytes of a vaccinia virus (VACV)-immune donor. To ascertain the complexity of the library, nucleotide sequences of the variable regions of t...

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Autores principales: Schmaljohn, Connie, Cui, Yunchang, Kerby, Stephen, Pennock, David, Spik, Kristin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 1999
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631611/
https://www.ncbi.nlm.nih.gov/pubmed/10329580
http://dx.doi.org/10.1006/viro.1999.9701
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author Schmaljohn, Connie
Cui, Yunchang
Kerby, Stephen
Pennock, David
Spik, Kristin
author_facet Schmaljohn, Connie
Cui, Yunchang
Kerby, Stephen
Pennock, David
Spik, Kristin
author_sort Schmaljohn, Connie
collection PubMed
description A combinatorial, phage-display library of human Fab antibody fragments was generated from IgG heavy chain (HC) and light chain (LC) genes cloned from the lymphocytes of a vaccinia virus (VACV)-immune donor. To ascertain the complexity of the library, nucleotide sequences of the variable regions of the HC and LC genes were determined. Fourteen distinct HC and 18 distinct LC (7 κ and 11 λ) that formed a combinatorial library of 22 Fabs were identified. Immune-precipitation of radiolabeled VACV revealed that at least six different VACV proteins were recognized by the antibodies. Plaque-reduction neutralization demonstrated that six of the Fabs neutralized VACV in the presence of anti-human antibody. ELISA studies indicated that 15 of the Fabs were cross-reactive with monkeypox virus.
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spelling pubmed-96316112022-11-03 Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library() Schmaljohn, Connie Cui, Yunchang Kerby, Stephen Pennock, David Spik, Kristin Virology Regular Article A combinatorial, phage-display library of human Fab antibody fragments was generated from IgG heavy chain (HC) and light chain (LC) genes cloned from the lymphocytes of a vaccinia virus (VACV)-immune donor. To ascertain the complexity of the library, nucleotide sequences of the variable regions of the HC and LC genes were determined. Fourteen distinct HC and 18 distinct LC (7 κ and 11 λ) that formed a combinatorial library of 22 Fabs were identified. Immune-precipitation of radiolabeled VACV revealed that at least six different VACV proteins were recognized by the antibodies. Plaque-reduction neutralization demonstrated that six of the Fabs neutralized VACV in the presence of anti-human antibody. ELISA studies indicated that 15 of the Fabs were cross-reactive with monkeypox virus. Published by Elsevier Inc. 1999-05-25 2002-05-25 /pmc/articles/PMC9631611/ /pubmed/10329580 http://dx.doi.org/10.1006/viro.1999.9701 Text en Copyright © 1999 Published by Elsevier Inc. Elsevier has created a Monkeypox Information Center (https://www.elsevier.com/connect/monkeypox-information-center) in response to the declared public health emergency of international concern, with free information in English on the monkeypox virus. The Monkeypox Information Center is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its monkeypox related research that is available on the Monkeypox Information Center - including this research content - immediately available in publicly funded repositories, with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the Monkeypox Information Center remains active.
spellingShingle Regular Article
Schmaljohn, Connie
Cui, Yunchang
Kerby, Stephen
Pennock, David
Spik, Kristin
Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title_full Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title_fullStr Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title_full_unstemmed Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title_short Production and Characterization of Human Monoclonal Antibody Fab Fragments to Vaccinia Virus from a Phage-Display Combinatorial Library()
title_sort production and characterization of human monoclonal antibody fab fragments to vaccinia virus from a phage-display combinatorial library()
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631611/
https://www.ncbi.nlm.nih.gov/pubmed/10329580
http://dx.doi.org/10.1006/viro.1999.9701
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