Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis

[Image: see text] In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG ant...

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Autores principales: Lin, Qiuyuan, Zhang, Jin, Liu, Liling, Kong, Jilie, Fang, Xueen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631717/
https://www.ncbi.nlm.nih.gov/pubmed/36340144
http://dx.doi.org/10.1021/acsomega.2c03499
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author Lin, Qiuyuan
Zhang, Jin
Liu, Liling
Kong, Jilie
Fang, Xueen
author_facet Lin, Qiuyuan
Zhang, Jin
Liu, Liling
Kong, Jilie
Fang, Xueen
author_sort Lin, Qiuyuan
collection PubMed
description [Image: see text] In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG antibody) with respective detection limits of 1 copy/μL for nucleic acid, 0.85 ng/mL for antigen, and 5.80 ng/mL for IgG within 30 min with high stability and anti-interference ability. The capability of this system in clinical applications was further evaluated using clinical samples, displaying 100% sensitivity and 100% specificity for COVID-19 diagnosis. These findings demonstrate the potential of this method to be used for the detection and subsequent control of pathogens.
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spelling pubmed-96317172022-11-04 Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis Lin, Qiuyuan Zhang, Jin Liu, Liling Kong, Jilie Fang, Xueen ACS Omega [Image: see text] In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG antibody) with respective detection limits of 1 copy/μL for nucleic acid, 0.85 ng/mL for antigen, and 5.80 ng/mL for IgG within 30 min with high stability and anti-interference ability. The capability of this system in clinical applications was further evaluated using clinical samples, displaying 100% sensitivity and 100% specificity for COVID-19 diagnosis. These findings demonstrate the potential of this method to be used for the detection and subsequent control of pathogens. American Chemical Society 2022-10-21 /pmc/articles/PMC9631717/ /pubmed/36340144 http://dx.doi.org/10.1021/acsomega.2c03499 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lin, Qiuyuan
Zhang, Jin
Liu, Liling
Kong, Jilie
Fang, Xueen
Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title_full Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title_fullStr Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title_full_unstemmed Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title_short Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis
title_sort simultaneous rapid nucleic acid and protein detection in a lateral chromatography chip for covid-19 diagnosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631717/
https://www.ncbi.nlm.nih.gov/pubmed/36340144
http://dx.doi.org/10.1021/acsomega.2c03499
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