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De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development

Chinavia impicticornis is a neotropical stink bug of economic importance for various crops. Little is known about the development of the species, or the genetic mechanisms that may favor the establishment of populations in cultivated plants. Here, we conduct the first large-scale molecular study of...

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Autores principales: Genevcius, Bruno C., Torres, Tatiana T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: GigaScience Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631951/
https://www.ncbi.nlm.nih.gov/pubmed/36824599
http://dx.doi.org/10.46471/gigabyte.11
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author Genevcius, Bruno C.
Torres, Tatiana T.
author_facet Genevcius, Bruno C.
Torres, Tatiana T.
author_sort Genevcius, Bruno C.
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description Chinavia impicticornis is a neotropical stink bug of economic importance for various crops. Little is known about the development of the species, or the genetic mechanisms that may favor the establishment of populations in cultivated plants. Here, we conduct the first large-scale molecular study of C. impicticornis. Using tissues derived from the genitalia and the rest of the body for two immature stages of both males and females, we generated RNA-seq data, then assembled and functionally annotated a transcriptome. The de novo-assembled transcriptome contained around 400,000 contigs, with an average length of 688 bp. After pruning duplicated sequences and conducting a functional annotation, the final annotated transcriptome comprised 39,478 transcripts, of which 12,665 were assigned to Gene Ontology (GO) terms. These novel datasets will be invaluable for the discovery of molecular processes related to morphogenesis and immature biology. We hope to contribute to the growing body of research on stink bug evolution and development, as well as to the development of biorational pest management solutions.
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spelling pubmed-96319512023-02-22 De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development Genevcius, Bruno C. Torres, Tatiana T. GigaByte Data Release Chinavia impicticornis is a neotropical stink bug of economic importance for various crops. Little is known about the development of the species, or the genetic mechanisms that may favor the establishment of populations in cultivated plants. Here, we conduct the first large-scale molecular study of C. impicticornis. Using tissues derived from the genitalia and the rest of the body for two immature stages of both males and females, we generated RNA-seq data, then assembled and functionally annotated a transcriptome. The de novo-assembled transcriptome contained around 400,000 contigs, with an average length of 688 bp. After pruning duplicated sequences and conducting a functional annotation, the final annotated transcriptome comprised 39,478 transcripts, of which 12,665 were assigned to Gene Ontology (GO) terms. These novel datasets will be invaluable for the discovery of molecular processes related to morphogenesis and immature biology. We hope to contribute to the growing body of research on stink bug evolution and development, as well as to the development of biorational pest management solutions. GigaScience Press 2020-12-31 /pmc/articles/PMC9631951/ /pubmed/36824599 http://dx.doi.org/10.46471/gigabyte.11 Text en © The Author(s) 2020. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Release
Genevcius, Bruno C.
Torres, Tatiana T.
De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title_full De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title_fullStr De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title_full_unstemmed De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title_short De novo construction of a transcriptome for the stink bug crop pest Chinavia impicticornis during late development
title_sort de novo construction of a transcriptome for the stink bug crop pest chinavia impicticornis during late development
topic Data Release
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631951/
https://www.ncbi.nlm.nih.gov/pubmed/36824599
http://dx.doi.org/10.46471/gigabyte.11
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