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Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)

Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni)...

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Autores principales: Duckett, Drew J., Sullivan, Jack, Pirro, Stacy, Carstens, Bryan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: GigaScience Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631978/
https://www.ncbi.nlm.nih.gov/pubmed/36824326
http://dx.doi.org/10.46471/gigabyte.19
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author Duckett, Drew J.
Sullivan, Jack
Pirro, Stacy
Carstens, Bryan C.
author_facet Duckett, Drew J.
Sullivan, Jack
Pirro, Stacy
Carstens, Bryan C.
author_sort Duckett, Drew J.
collection PubMed
description Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus Microtus, making the water vole and montane vole genomes useful additions to currently available genomic resources.
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spelling pubmed-96319782023-02-22 Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus) Duckett, Drew J. Sullivan, Jack Pirro, Stacy Carstens, Bryan C. GigaByte Data Release Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus Microtus, making the water vole and montane vole genomes useful additions to currently available genomic resources. GigaScience Press 2021-05-06 /pmc/articles/PMC9631978/ /pubmed/36824326 http://dx.doi.org/10.46471/gigabyte.19 Text en © The Author(s) 2021. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Release
Duckett, Drew J.
Sullivan, Jack
Pirro, Stacy
Carstens, Bryan C.
Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_full Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_fullStr Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_full_unstemmed Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_short Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_sort genomic resources for the north american water vole (microtus richardsoni) and the montane vole (microtus montanus)
topic Data Release
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631978/
https://www.ncbi.nlm.nih.gov/pubmed/36824326
http://dx.doi.org/10.46471/gigabyte.19
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