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Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes

[Image: see text] Wastewater-based surveillance (WBS) for disease monitoring is highly promising but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. Herein, we describe a comprehensive metagenomics-based approach for global surveillance of antibioti...

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Autores principales: Prieto Riquelme, Maria Virginia, Garner, Emily, Gupta, Suraj, Metch, Jake, Zhu, Ni, Blair, Matthew F., Arango-Argoty, Gustavo, Maile-Moskowitz, Ayella, Li, An-dong, Flach, Carl-Fredrik, Aga, Diana S., Nambi, Indumathi M., Larsson, D. G. Joakim, Bürgmann, Helmut, Zhang, Tong, Pruden, Amy, Vikesland, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631994/
https://www.ncbi.nlm.nih.gov/pubmed/35759608
http://dx.doi.org/10.1021/acs.est.1c08673
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author Prieto Riquelme, Maria Virginia
Garner, Emily
Gupta, Suraj
Metch, Jake
Zhu, Ni
Blair, Matthew F.
Arango-Argoty, Gustavo
Maile-Moskowitz, Ayella
Li, An-dong
Flach, Carl-Fredrik
Aga, Diana S.
Nambi, Indumathi M.
Larsson, D. G. Joakim
Bürgmann, Helmut
Zhang, Tong
Pruden, Amy
Vikesland, Peter J.
author_facet Prieto Riquelme, Maria Virginia
Garner, Emily
Gupta, Suraj
Metch, Jake
Zhu, Ni
Blair, Matthew F.
Arango-Argoty, Gustavo
Maile-Moskowitz, Ayella
Li, An-dong
Flach, Carl-Fredrik
Aga, Diana S.
Nambi, Indumathi M.
Larsson, D. G. Joakim
Bürgmann, Helmut
Zhang, Tong
Pruden, Amy
Vikesland, Peter J.
author_sort Prieto Riquelme, Maria Virginia
collection PubMed
description [Image: see text] Wastewater-based surveillance (WBS) for disease monitoring is highly promising but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. Herein, we describe a comprehensive metagenomics-based approach for global surveillance of antibiotic resistance in sewage that enables assessment of 1) which antibiotic resistance genes (ARGs) are shared across regions/communities; 2) which ARGs are discriminatory; and 3) factors associated with overall trends in ARGs, such as antibiotic concentrations. Across an internationally sourced transect of sewage samples collected using a centralized, standardized protocol, ARG relative abundances (16S rRNA gene-normalized) were highest in Hong Kong and India and lowest in Sweden and Switzerland, reflecting national policy, measured antibiotic concentrations, and metal resistance genes. Asian versus European/US resistomes were distinct, with macrolide-lincosamide-streptogramin, phenicol, quinolone, and tetracycline versus multidrug resistance ARGs being discriminatory, respectively. Regional trends in measured antibiotic concentrations differed from trends expected from public sales data. This could reflect unaccounted uses, captured only by the WBS approach. If properly benchmarked, antibiotic WBS might complement public sales and consumption statistics in the future. The WBS approach defined herein demonstrates multisite comparability and sensitivity to local/regional factors.
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spelling pubmed-96319942022-11-04 Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes Prieto Riquelme, Maria Virginia Garner, Emily Gupta, Suraj Metch, Jake Zhu, Ni Blair, Matthew F. Arango-Argoty, Gustavo Maile-Moskowitz, Ayella Li, An-dong Flach, Carl-Fredrik Aga, Diana S. Nambi, Indumathi M. Larsson, D. G. Joakim Bürgmann, Helmut Zhang, Tong Pruden, Amy Vikesland, Peter J. Environ Sci Technol [Image: see text] Wastewater-based surveillance (WBS) for disease monitoring is highly promising but requires consistent methodologies that incorporate predetermined objectives, targets, and metrics. Herein, we describe a comprehensive metagenomics-based approach for global surveillance of antibiotic resistance in sewage that enables assessment of 1) which antibiotic resistance genes (ARGs) are shared across regions/communities; 2) which ARGs are discriminatory; and 3) factors associated with overall trends in ARGs, such as antibiotic concentrations. Across an internationally sourced transect of sewage samples collected using a centralized, standardized protocol, ARG relative abundances (16S rRNA gene-normalized) were highest in Hong Kong and India and lowest in Sweden and Switzerland, reflecting national policy, measured antibiotic concentrations, and metal resistance genes. Asian versus European/US resistomes were distinct, with macrolide-lincosamide-streptogramin, phenicol, quinolone, and tetracycline versus multidrug resistance ARGs being discriminatory, respectively. Regional trends in measured antibiotic concentrations differed from trends expected from public sales data. This could reflect unaccounted uses, captured only by the WBS approach. If properly benchmarked, antibiotic WBS might complement public sales and consumption statistics in the future. The WBS approach defined herein demonstrates multisite comparability and sensitivity to local/regional factors. American Chemical Society 2022-06-27 2022-11-01 /pmc/articles/PMC9631994/ /pubmed/35759608 http://dx.doi.org/10.1021/acs.est.1c08673 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Prieto Riquelme, Maria Virginia
Garner, Emily
Gupta, Suraj
Metch, Jake
Zhu, Ni
Blair, Matthew F.
Arango-Argoty, Gustavo
Maile-Moskowitz, Ayella
Li, An-dong
Flach, Carl-Fredrik
Aga, Diana S.
Nambi, Indumathi M.
Larsson, D. G. Joakim
Bürgmann, Helmut
Zhang, Tong
Pruden, Amy
Vikesland, Peter J.
Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title_full Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title_fullStr Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title_full_unstemmed Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title_short Demonstrating a Comprehensive Wastewater-Based Surveillance Approach That Differentiates Globally Sourced Resistomes
title_sort demonstrating a comprehensive wastewater-based surveillance approach that differentiates globally sourced resistomes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631994/
https://www.ncbi.nlm.nih.gov/pubmed/35759608
http://dx.doi.org/10.1021/acs.est.1c08673
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