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Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632490/ https://www.ncbi.nlm.nih.gov/pubmed/36339146 http://dx.doi.org/10.3389/fpubh.2022.987517 |
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author | Zhang, Liwen Lv, Qingyu Zheng, Yuling Gao, Shan Huang, Wenhua Liu, Peng Kong, Decong Wang, Ye Yu, Yunzhou Jiang, Yongqiang Jiang, Hua |
author_facet | Zhang, Liwen Lv, Qingyu Zheng, Yuling Gao, Shan Huang, Wenhua Liu, Peng Kong, Decong Wang, Ye Yu, Yunzhou Jiang, Yongqiang Jiang, Hua |
author_sort | Zhang, Liwen |
collection | PubMed |
description | Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous dose of botulinum toxin exceptionally low and intravenous human lethal doses estimated at 1-3 ng/kg. Therefore, sensitive and accurate detection methods suitable for testing a wide range of complex samples are urgently needed. To this end, the “amplified luminescent proximity homogeneous assay linked immunosorbent assay” (AlphaLISA) was established for the detection of BoNT/A and its detection efficacy in plasma, beverage, food, and other complex samples was evaluated. The results showed that this method can very effectively resist matrix interference. The detection time is rapid, reaching a detection limit for all samples of up to 0.1 ng/mL in only 30 min. BoNT/A can also be accurately detected in vomit samples of patients with clinical food poisoning. This study demonstrates that AlphaLISA is an effective tool for the detection of BoNT/A in complex samples and can potentially be developed for commercial use in the future. |
format | Online Article Text |
id | pubmed-9632490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96324902022-11-04 Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA Zhang, Liwen Lv, Qingyu Zheng, Yuling Gao, Shan Huang, Wenhua Liu, Peng Kong, Decong Wang, Ye Yu, Yunzhou Jiang, Yongqiang Jiang, Hua Front Public Health Public Health Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous dose of botulinum toxin exceptionally low and intravenous human lethal doses estimated at 1-3 ng/kg. Therefore, sensitive and accurate detection methods suitable for testing a wide range of complex samples are urgently needed. To this end, the “amplified luminescent proximity homogeneous assay linked immunosorbent assay” (AlphaLISA) was established for the detection of BoNT/A and its detection efficacy in plasma, beverage, food, and other complex samples was evaluated. The results showed that this method can very effectively resist matrix interference. The detection time is rapid, reaching a detection limit for all samples of up to 0.1 ng/mL in only 30 min. BoNT/A can also be accurately detected in vomit samples of patients with clinical food poisoning. This study demonstrates that AlphaLISA is an effective tool for the detection of BoNT/A in complex samples and can potentially be developed for commercial use in the future. Frontiers Media S.A. 2022-10-20 /pmc/articles/PMC9632490/ /pubmed/36339146 http://dx.doi.org/10.3389/fpubh.2022.987517 Text en Copyright © 2022 Zhang, Lv, Zheng, Gao, Huang, Liu, Kong, Wang, Yu, Jiang and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Public Health Zhang, Liwen Lv, Qingyu Zheng, Yuling Gao, Shan Huang, Wenhua Liu, Peng Kong, Decong Wang, Ye Yu, Yunzhou Jiang, Yongqiang Jiang, Hua Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title | Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title_full | Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title_fullStr | Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title_full_unstemmed | Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title_short | Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA |
title_sort | rapid and sensitive detection of botulinum toxin type a in complex sample matrices by alphalisa |
topic | Public Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632490/ https://www.ncbi.nlm.nih.gov/pubmed/36339146 http://dx.doi.org/10.3389/fpubh.2022.987517 |
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