Cargando…

Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA

Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Liwen, Lv, Qingyu, Zheng, Yuling, Gao, Shan, Huang, Wenhua, Liu, Peng, Kong, Decong, Wang, Ye, Yu, Yunzhou, Jiang, Yongqiang, Jiang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632490/
https://www.ncbi.nlm.nih.gov/pubmed/36339146
http://dx.doi.org/10.3389/fpubh.2022.987517
_version_ 1784824038793674752
author Zhang, Liwen
Lv, Qingyu
Zheng, Yuling
Gao, Shan
Huang, Wenhua
Liu, Peng
Kong, Decong
Wang, Ye
Yu, Yunzhou
Jiang, Yongqiang
Jiang, Hua
author_facet Zhang, Liwen
Lv, Qingyu
Zheng, Yuling
Gao, Shan
Huang, Wenhua
Liu, Peng
Kong, Decong
Wang, Ye
Yu, Yunzhou
Jiang, Yongqiang
Jiang, Hua
author_sort Zhang, Liwen
collection PubMed
description Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous dose of botulinum toxin exceptionally low and intravenous human lethal doses estimated at 1-3 ng/kg. Therefore, sensitive and accurate detection methods suitable for testing a wide range of complex samples are urgently needed. To this end, the “amplified luminescent proximity homogeneous assay linked immunosorbent assay” (AlphaLISA) was established for the detection of BoNT/A and its detection efficacy in plasma, beverage, food, and other complex samples was evaluated. The results showed that this method can very effectively resist matrix interference. The detection time is rapid, reaching a detection limit for all samples of up to 0.1 ng/mL in only 30 min. BoNT/A can also be accurately detected in vomit samples of patients with clinical food poisoning. This study demonstrates that AlphaLISA is an effective tool for the detection of BoNT/A in complex samples and can potentially be developed for commercial use in the future.
format Online
Article
Text
id pubmed-9632490
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96324902022-11-04 Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA Zhang, Liwen Lv, Qingyu Zheng, Yuling Gao, Shan Huang, Wenhua Liu, Peng Kong, Decong Wang, Ye Yu, Yunzhou Jiang, Yongqiang Jiang, Hua Front Public Health Public Health Botulinum toxin A(BoNT/A) is a neurotoxin produced by the bacteria Clostridium botulinum, which can cause serious food poisoning and is recognized as a potential biological warfare agent. BoNT/A is does not degrade easily and can remain in the complex matrix for a long time. Meanwhile, the poisonous dose of botulinum toxin exceptionally low and intravenous human lethal doses estimated at 1-3 ng/kg. Therefore, sensitive and accurate detection methods suitable for testing a wide range of complex samples are urgently needed. To this end, the “amplified luminescent proximity homogeneous assay linked immunosorbent assay” (AlphaLISA) was established for the detection of BoNT/A and its detection efficacy in plasma, beverage, food, and other complex samples was evaluated. The results showed that this method can very effectively resist matrix interference. The detection time is rapid, reaching a detection limit for all samples of up to 0.1 ng/mL in only 30 min. BoNT/A can also be accurately detected in vomit samples of patients with clinical food poisoning. This study demonstrates that AlphaLISA is an effective tool for the detection of BoNT/A in complex samples and can potentially be developed for commercial use in the future. Frontiers Media S.A. 2022-10-20 /pmc/articles/PMC9632490/ /pubmed/36339146 http://dx.doi.org/10.3389/fpubh.2022.987517 Text en Copyright © 2022 Zhang, Lv, Zheng, Gao, Huang, Liu, Kong, Wang, Yu, Jiang and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Zhang, Liwen
Lv, Qingyu
Zheng, Yuling
Gao, Shan
Huang, Wenhua
Liu, Peng
Kong, Decong
Wang, Ye
Yu, Yunzhou
Jiang, Yongqiang
Jiang, Hua
Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title_full Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title_fullStr Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title_full_unstemmed Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title_short Rapid and sensitive detection of botulinum toxin type A in complex sample matrices by AlphaLISA
title_sort rapid and sensitive detection of botulinum toxin type a in complex sample matrices by alphalisa
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632490/
https://www.ncbi.nlm.nih.gov/pubmed/36339146
http://dx.doi.org/10.3389/fpubh.2022.987517
work_keys_str_mv AT zhangliwen rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT lvqingyu rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT zhengyuling rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT gaoshan rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT huangwenhua rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT liupeng rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT kongdecong rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT wangye rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT yuyunzhou rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT jiangyongqiang rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa
AT jianghua rapidandsensitivedetectionofbotulinumtoxintypeaincomplexsamplematricesbyalphalisa