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Fabrication of microtiter plate on paper using 96-well plates for wax stamping
Paper-based analytical devices have prominently emerged as a group of diagnostic tools with prospective to eliminate the expensive, time-consuming, and intricate analytical methodologies. Wax printing has been a dominant technique to fabricate hydrophobic patterns on paper for fluid control, but the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632569/ https://www.ncbi.nlm.nih.gov/pubmed/36349227 http://dx.doi.org/10.1007/s10404-022-02606-3 |
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author | Borah, Madhurima Maheswari, Diksha Dutta, Hemant Sankar |
author_facet | Borah, Madhurima Maheswari, Diksha Dutta, Hemant Sankar |
author_sort | Borah, Madhurima |
collection | PubMed |
description | Paper-based analytical devices have prominently emerged as a group of diagnostic tools with prospective to eliminate the expensive, time-consuming, and intricate analytical methodologies. Wax printing has been a dominant technique to fabricate hydrophobic patterns on paper for fluid control, but the discontinuation of commercial solid ink printers has begun a genesis of alternate wax patterning strategies. In this study, a simple and rapid fabrication methodology for realizing a 96-well microtiter plate on paper has been developed. The method involves the use of commercially available polystyrene microplates as a stamp for wax patterning. The technique further eradicates the requirement of customized stamps and the step of heating paper substrates for creating wax barriers. Thus, wax stamped paper microplates can be used for a wide range of bioanalytical tests maneuvering reduced generation of non-biodegradable waste, minimal reagent usage, and inexpensive readout strategies. The viability of the fabricated platform has been assessed by colorimetric detection of glutathione using 3,3′,5,5′-tetramethylbenzidine–H(2)O(2) redox system. RGB analysis of the colorimetric response showed a linear concentration range from 0 to 90 µM (R(2) = 0.989) along with a detection limit of 28.375 µM. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10404-022-02606-3. |
format | Online Article Text |
id | pubmed-9632569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-96325692022-11-04 Fabrication of microtiter plate on paper using 96-well plates for wax stamping Borah, Madhurima Maheswari, Diksha Dutta, Hemant Sankar Microfluid Nanofluidics Research Paper Paper-based analytical devices have prominently emerged as a group of diagnostic tools with prospective to eliminate the expensive, time-consuming, and intricate analytical methodologies. Wax printing has been a dominant technique to fabricate hydrophobic patterns on paper for fluid control, but the discontinuation of commercial solid ink printers has begun a genesis of alternate wax patterning strategies. In this study, a simple and rapid fabrication methodology for realizing a 96-well microtiter plate on paper has been developed. The method involves the use of commercially available polystyrene microplates as a stamp for wax patterning. The technique further eradicates the requirement of customized stamps and the step of heating paper substrates for creating wax barriers. Thus, wax stamped paper microplates can be used for a wide range of bioanalytical tests maneuvering reduced generation of non-biodegradable waste, minimal reagent usage, and inexpensive readout strategies. The viability of the fabricated platform has been assessed by colorimetric detection of glutathione using 3,3′,5,5′-tetramethylbenzidine–H(2)O(2) redox system. RGB analysis of the colorimetric response showed a linear concentration range from 0 to 90 µM (R(2) = 0.989) along with a detection limit of 28.375 µM. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10404-022-02606-3. Springer Berlin Heidelberg 2022-11-03 2022 /pmc/articles/PMC9632569/ /pubmed/36349227 http://dx.doi.org/10.1007/s10404-022-02606-3 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Paper Borah, Madhurima Maheswari, Diksha Dutta, Hemant Sankar Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title | Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title_full | Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title_fullStr | Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title_full_unstemmed | Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title_short | Fabrication of microtiter plate on paper using 96-well plates for wax stamping |
title_sort | fabrication of microtiter plate on paper using 96-well plates for wax stamping |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632569/ https://www.ncbi.nlm.nih.gov/pubmed/36349227 http://dx.doi.org/10.1007/s10404-022-02606-3 |
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