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Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome

Growing evidence has indicated that the characteristics of gut microbiota are associated with acute ischemic stroke (AIS). Phlegm-heat syndrome (PHS), a specific pathological state of the AIS, is one of the common traditional Chinese syndromes of stroke. The long duration of PHS in patients with AIS...

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Autores principales: Li, Tingting, Sun, Qianhui, Feng, Luda, Yan, Dong, Wang, Boyuan, Li, Mingxuan, Xiong, Xuejiao, Ma, Dayong, Gao, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632779/
https://www.ncbi.nlm.nih.gov/pubmed/36327217
http://dx.doi.org/10.1371/journal.pone.0276598
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author Li, Tingting
Sun, Qianhui
Feng, Luda
Yan, Dong
Wang, Boyuan
Li, Mingxuan
Xiong, Xuejiao
Ma, Dayong
Gao, Ying
author_facet Li, Tingting
Sun, Qianhui
Feng, Luda
Yan, Dong
Wang, Boyuan
Li, Mingxuan
Xiong, Xuejiao
Ma, Dayong
Gao, Ying
author_sort Li, Tingting
collection PubMed
description Growing evidence has indicated that the characteristics of gut microbiota are associated with acute ischemic stroke (AIS). Phlegm-heat syndrome (PHS), a specific pathological state of the AIS, is one of the common traditional Chinese syndromes of stroke. The long duration of PHS in patients with AIS could lead to poor clinical outcomes. Gut microbiota characteristics in patients with both AIS and PHS, and their relationship remains unknown. This study was designed to investigate the alterations in gut microbiota in patients with AIS and PHS through a cross-sectional study. Fecal samples were collected from 10 patients with AIS and non-PHS (ntAIS), 7 patients with AIS and PHS (tAIS), and 10 healthy controls (HC). Samples were profiled via Illumina sequencing of the 16S rRNA V3-V4. Stroke severity was assessed at admission by the National Institutes of Health Stroke Scale (NIHSS) and modified Rankin scale (mRS); their correlation with gut microbiota was investigated. The alpha-diversity of the bacterial communities was significantly higher in the fecal samples of patients with tAIS than in patients with ntAIS (Shannon index, P = 0.037). In addition, the combined tAIS and ntAIS group (tntAIS) exhibited higher microbiotic diversity when compared with HC (chao1, P = 0.019). The structure of intestinal microbiota was effectively distinguished between the tAIS and ntAIS group (ANOSIM, r = 0.337, P = 0.007). Additionally, the gut microbiota structure was significantly different between the tntAIS and HC groups (ANOSIM, r = 0.217, P = 0.005). The genera, Ruminococcaceae_ UCG_002 and Christensenellaceae_R-7_group, were implicated in the discrimination of PHS from non-PHS. The order Lactobacillales and family Lachnospiraceae were significantly negatively correlated with NIHSS and mRS at admission (P < 0.05). By contrast, the order Desulfovibrionales, families Christensenellaceae and Desulfovibrionaceae, and genera Ruminococcaceae UCG-014 and Ruminococcaceae UCG-002 were significantly positively correlated with NIHSS and mRS at admission (P < 0.05). This study is the first to profile the characteristics of gut microbiota in patients with AIS and PHS, compared with those with non-PHS. The genera, Ruminococcaceae_ UCG_002 and Christensenellaceae_R-7_group, may be objective indicators of this traditional Chinese medicine (TCM) syndrome in AIS. Furthermore, it provides a microbe-inspired biological basis for TCM syndrome differentiation.
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spelling pubmed-96327792022-11-04 Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome Li, Tingting Sun, Qianhui Feng, Luda Yan, Dong Wang, Boyuan Li, Mingxuan Xiong, Xuejiao Ma, Dayong Gao, Ying PLoS One Research Article Growing evidence has indicated that the characteristics of gut microbiota are associated with acute ischemic stroke (AIS). Phlegm-heat syndrome (PHS), a specific pathological state of the AIS, is one of the common traditional Chinese syndromes of stroke. The long duration of PHS in patients with AIS could lead to poor clinical outcomes. Gut microbiota characteristics in patients with both AIS and PHS, and their relationship remains unknown. This study was designed to investigate the alterations in gut microbiota in patients with AIS and PHS through a cross-sectional study. Fecal samples were collected from 10 patients with AIS and non-PHS (ntAIS), 7 patients with AIS and PHS (tAIS), and 10 healthy controls (HC). Samples were profiled via Illumina sequencing of the 16S rRNA V3-V4. Stroke severity was assessed at admission by the National Institutes of Health Stroke Scale (NIHSS) and modified Rankin scale (mRS); their correlation with gut microbiota was investigated. The alpha-diversity of the bacterial communities was significantly higher in the fecal samples of patients with tAIS than in patients with ntAIS (Shannon index, P = 0.037). In addition, the combined tAIS and ntAIS group (tntAIS) exhibited higher microbiotic diversity when compared with HC (chao1, P = 0.019). The structure of intestinal microbiota was effectively distinguished between the tAIS and ntAIS group (ANOSIM, r = 0.337, P = 0.007). Additionally, the gut microbiota structure was significantly different between the tntAIS and HC groups (ANOSIM, r = 0.217, P = 0.005). The genera, Ruminococcaceae_ UCG_002 and Christensenellaceae_R-7_group, were implicated in the discrimination of PHS from non-PHS. The order Lactobacillales and family Lachnospiraceae were significantly negatively correlated with NIHSS and mRS at admission (P < 0.05). By contrast, the order Desulfovibrionales, families Christensenellaceae and Desulfovibrionaceae, and genera Ruminococcaceae UCG-014 and Ruminococcaceae UCG-002 were significantly positively correlated with NIHSS and mRS at admission (P < 0.05). This study is the first to profile the characteristics of gut microbiota in patients with AIS and PHS, compared with those with non-PHS. The genera, Ruminococcaceae_ UCG_002 and Christensenellaceae_R-7_group, may be objective indicators of this traditional Chinese medicine (TCM) syndrome in AIS. Furthermore, it provides a microbe-inspired biological basis for TCM syndrome differentiation. Public Library of Science 2022-11-03 /pmc/articles/PMC9632779/ /pubmed/36327217 http://dx.doi.org/10.1371/journal.pone.0276598 Text en © 2022 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Tingting
Sun, Qianhui
Feng, Luda
Yan, Dong
Wang, Boyuan
Li, Mingxuan
Xiong, Xuejiao
Ma, Dayong
Gao, Ying
Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title_full Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title_fullStr Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title_full_unstemmed Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title_short Uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
title_sort uncovering the characteristics of the gut microbiota in patients with acute ischemic stroke and phlegm-heat syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632779/
https://www.ncbi.nlm.nih.gov/pubmed/36327217
http://dx.doi.org/10.1371/journal.pone.0276598
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