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A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana

Organelles contribute to plant growth via their movements and interactions, which ensure efficient metabolic flow and help plants adapt to environmental stress. Live-cell imaging of the interactions of organelles has been performed in yeast, plant, and animal cells. However, high-throughput quantita...

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Autores principales: Oikawa, Kazusato, Midorikawa, Keiko, Kodama, Yutaka, Numata, Keiji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632854/
https://www.ncbi.nlm.nih.gov/pubmed/36340392
http://dx.doi.org/10.3389/fpls.2022.998960
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author Oikawa, Kazusato
Midorikawa, Keiko
Kodama, Yutaka
Numata, Keiji
author_facet Oikawa, Kazusato
Midorikawa, Keiko
Kodama, Yutaka
Numata, Keiji
author_sort Oikawa, Kazusato
collection PubMed
description Organelles contribute to plant growth via their movements and interactions, which ensure efficient metabolic flow and help plants adapt to environmental stress. Live-cell imaging of the interactions of organelles has been performed in yeast, plant, and animal cells. However, high-throughput quantitative methods are needed to simultaneously analyze the interactions of many organelles in living plant cells. Here, we developed a semi-automatic high-throughput method to quantitatively evaluate the interactions between peroxisomes and chloroplasts using a distance transformation algorithm and high-resolution 3D fluorescent images taken by confocal laser scanning microscopy. Using this method, we measured the 3D distance between the center of peroxisome and chloroplast surface in Arabidopsis thaliana. We then compared the distances between these organelles in leaf mesophyll cells under light and dark conditions. This distance was shorter in the light than in the dark, which is in agreement with the findings of previous studies. We used our method to evaluate peroxisome–chloroplast (plastid) interactions in different cell types in the light and dark, including guard, stem, and root cells. Like in mesophyll cells, the distance between the peroxisome and chloroplast was shorter in the light in guard and stem cells, but not in root cells, suggesting that photosynthetic plastids (chloroplasts) play important roles in these interactions. When leaf mesophyll cells were incubated under high-intensity light, the frequency of shorter distances between peroxisomes and chloroplasts significantly increased. Our high-throughput, semi-automatic method represents a powerful tool for evaluating peroxisome–chloroplast interactions in different types of plant cells under various environmental conditions.
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spelling pubmed-96328542022-11-04 A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana Oikawa, Kazusato Midorikawa, Keiko Kodama, Yutaka Numata, Keiji Front Plant Sci Plant Science Organelles contribute to plant growth via their movements and interactions, which ensure efficient metabolic flow and help plants adapt to environmental stress. Live-cell imaging of the interactions of organelles has been performed in yeast, plant, and animal cells. However, high-throughput quantitative methods are needed to simultaneously analyze the interactions of many organelles in living plant cells. Here, we developed a semi-automatic high-throughput method to quantitatively evaluate the interactions between peroxisomes and chloroplasts using a distance transformation algorithm and high-resolution 3D fluorescent images taken by confocal laser scanning microscopy. Using this method, we measured the 3D distance between the center of peroxisome and chloroplast surface in Arabidopsis thaliana. We then compared the distances between these organelles in leaf mesophyll cells under light and dark conditions. This distance was shorter in the light than in the dark, which is in agreement with the findings of previous studies. We used our method to evaluate peroxisome–chloroplast (plastid) interactions in different cell types in the light and dark, including guard, stem, and root cells. Like in mesophyll cells, the distance between the peroxisome and chloroplast was shorter in the light in guard and stem cells, but not in root cells, suggesting that photosynthetic plastids (chloroplasts) play important roles in these interactions. When leaf mesophyll cells were incubated under high-intensity light, the frequency of shorter distances between peroxisomes and chloroplasts significantly increased. Our high-throughput, semi-automatic method represents a powerful tool for evaluating peroxisome–chloroplast interactions in different types of plant cells under various environmental conditions. Frontiers Media S.A. 2022-10-20 /pmc/articles/PMC9632854/ /pubmed/36340392 http://dx.doi.org/10.3389/fpls.2022.998960 Text en Copyright © 2022 Oikawa, Midorikawa, Kodama and Numata https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Oikawa, Kazusato
Midorikawa, Keiko
Kodama, Yutaka
Numata, Keiji
A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title_full A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title_fullStr A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title_full_unstemmed A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title_short A high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in Arabidopsis thaliana
title_sort high-throughput quantitative method to evaluate peroxisome-chloroplast interactions in arabidopsis thaliana
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9632854/
https://www.ncbi.nlm.nih.gov/pubmed/36340392
http://dx.doi.org/10.3389/fpls.2022.998960
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