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Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. He...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633003/ https://www.ncbi.nlm.nih.gov/pubmed/36256684 http://dx.doi.org/10.1371/journal.ppat.1010909 |
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author | Wang, Liping Tan, Huang Medina-Puche, Laura Wu, Mengshi Garnelo Gomez, Borja Gao, Man Shi, Chaonan Jimenez-Gongora, Tamara Fan, Pengfei Ding, Xue Zhang, Dan Ding, Yi Rosas-Díaz, Tábata Liu, Yujing Aguilar, Emmanuel Fu, Xing Lozano-Durán, Rosa |
author_facet | Wang, Liping Tan, Huang Medina-Puche, Laura Wu, Mengshi Garnelo Gomez, Borja Gao, Man Shi, Chaonan Jimenez-Gongora, Tamara Fan, Pengfei Ding, Xue Zhang, Dan Ding, Yi Rosas-Díaz, Tábata Liu, Yujing Aguilar, Emmanuel Fu, Xing Lozano-Durán, Rosa |
author_sort | Wang, Liping |
collection | PubMed |
description | Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome. We show that the proteins encoded by a plant-infecting DNA virus, the geminivirus tomato yellow leaf curl virus (TYLCV), physically associate with one another in an intricate network, as detected by a number of protein-protein interaction techniques. Importantly, our results indicate that intra-viral protein-protein interactions can modify the subcellular localization of the proteins involved. Using one particular pairwise interaction, that between the virus-encoded C2 and CP proteins, as proof-of-concept, we demonstrate that the combination of viral proteins leads to novel transcriptional effects on the host cell. Taken together, our results underscore the importance of studying viral protein function in the context of the infection. We propose a model in which viral proteins might have evolved to extensively interact with other elements within the viral proteome, enlarging the potential functional landscape available to the pathogen. |
format | Online Article Text |
id | pubmed-9633003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-96330032022-11-04 Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome Wang, Liping Tan, Huang Medina-Puche, Laura Wu, Mengshi Garnelo Gomez, Borja Gao, Man Shi, Chaonan Jimenez-Gongora, Tamara Fan, Pengfei Ding, Xue Zhang, Dan Ding, Yi Rosas-Díaz, Tábata Liu, Yujing Aguilar, Emmanuel Fu, Xing Lozano-Durán, Rosa PLoS Pathog Research Article Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome. We show that the proteins encoded by a plant-infecting DNA virus, the geminivirus tomato yellow leaf curl virus (TYLCV), physically associate with one another in an intricate network, as detected by a number of protein-protein interaction techniques. Importantly, our results indicate that intra-viral protein-protein interactions can modify the subcellular localization of the proteins involved. Using one particular pairwise interaction, that between the virus-encoded C2 and CP proteins, as proof-of-concept, we demonstrate that the combination of viral proteins leads to novel transcriptional effects on the host cell. Taken together, our results underscore the importance of studying viral protein function in the context of the infection. We propose a model in which viral proteins might have evolved to extensively interact with other elements within the viral proteome, enlarging the potential functional landscape available to the pathogen. Public Library of Science 2022-10-18 /pmc/articles/PMC9633003/ /pubmed/36256684 http://dx.doi.org/10.1371/journal.ppat.1010909 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Liping Tan, Huang Medina-Puche, Laura Wu, Mengshi Garnelo Gomez, Borja Gao, Man Shi, Chaonan Jimenez-Gongora, Tamara Fan, Pengfei Ding, Xue Zhang, Dan Ding, Yi Rosas-Díaz, Tábata Liu, Yujing Aguilar, Emmanuel Fu, Xing Lozano-Durán, Rosa Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title | Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title_full | Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title_fullStr | Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title_full_unstemmed | Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title_short | Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
title_sort | combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633003/ https://www.ncbi.nlm.nih.gov/pubmed/36256684 http://dx.doi.org/10.1371/journal.ppat.1010909 |
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