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Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome

Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. He...

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Autores principales: Wang, Liping, Tan, Huang, Medina-Puche, Laura, Wu, Mengshi, Garnelo Gomez, Borja, Gao, Man, Shi, Chaonan, Jimenez-Gongora, Tamara, Fan, Pengfei, Ding, Xue, Zhang, Dan, Ding, Yi, Rosas-Díaz, Tábata, Liu, Yujing, Aguilar, Emmanuel, Fu, Xing, Lozano-Durán, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633003/
https://www.ncbi.nlm.nih.gov/pubmed/36256684
http://dx.doi.org/10.1371/journal.ppat.1010909
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author Wang, Liping
Tan, Huang
Medina-Puche, Laura
Wu, Mengshi
Garnelo Gomez, Borja
Gao, Man
Shi, Chaonan
Jimenez-Gongora, Tamara
Fan, Pengfei
Ding, Xue
Zhang, Dan
Ding, Yi
Rosas-Díaz, Tábata
Liu, Yujing
Aguilar, Emmanuel
Fu, Xing
Lozano-Durán, Rosa
author_facet Wang, Liping
Tan, Huang
Medina-Puche, Laura
Wu, Mengshi
Garnelo Gomez, Borja
Gao, Man
Shi, Chaonan
Jimenez-Gongora, Tamara
Fan, Pengfei
Ding, Xue
Zhang, Dan
Ding, Yi
Rosas-Díaz, Tábata
Liu, Yujing
Aguilar, Emmanuel
Fu, Xing
Lozano-Durán, Rosa
author_sort Wang, Liping
collection PubMed
description Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome. We show that the proteins encoded by a plant-infecting DNA virus, the geminivirus tomato yellow leaf curl virus (TYLCV), physically associate with one another in an intricate network, as detected by a number of protein-protein interaction techniques. Importantly, our results indicate that intra-viral protein-protein interactions can modify the subcellular localization of the proteins involved. Using one particular pairwise interaction, that between the virus-encoded C2 and CP proteins, as proof-of-concept, we demonstrate that the combination of viral proteins leads to novel transcriptional effects on the host cell. Taken together, our results underscore the importance of studying viral protein function in the context of the infection. We propose a model in which viral proteins might have evolved to extensively interact with other elements within the viral proteome, enlarging the potential functional landscape available to the pathogen.
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spelling pubmed-96330032022-11-04 Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome Wang, Liping Tan, Huang Medina-Puche, Laura Wu, Mengshi Garnelo Gomez, Borja Gao, Man Shi, Chaonan Jimenez-Gongora, Tamara Fan, Pengfei Ding, Xue Zhang, Dan Ding, Yi Rosas-Díaz, Tábata Liu, Yujing Aguilar, Emmanuel Fu, Xing Lozano-Durán, Rosa PLoS Pathog Research Article Viruses manipulate the cells they infect in order to replicate and spread. Due to strict size restrictions, viral genomes have reduced genetic space; how the action of the limited number of viral proteins results in the cell reprogramming observed during the infection is a long-standing question. Here, we explore the hypothesis that combinatorial interactions may expand the functional landscape of the viral proteome. We show that the proteins encoded by a plant-infecting DNA virus, the geminivirus tomato yellow leaf curl virus (TYLCV), physically associate with one another in an intricate network, as detected by a number of protein-protein interaction techniques. Importantly, our results indicate that intra-viral protein-protein interactions can modify the subcellular localization of the proteins involved. Using one particular pairwise interaction, that between the virus-encoded C2 and CP proteins, as proof-of-concept, we demonstrate that the combination of viral proteins leads to novel transcriptional effects on the host cell. Taken together, our results underscore the importance of studying viral protein function in the context of the infection. We propose a model in which viral proteins might have evolved to extensively interact with other elements within the viral proteome, enlarging the potential functional landscape available to the pathogen. Public Library of Science 2022-10-18 /pmc/articles/PMC9633003/ /pubmed/36256684 http://dx.doi.org/10.1371/journal.ppat.1010909 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Liping
Tan, Huang
Medina-Puche, Laura
Wu, Mengshi
Garnelo Gomez, Borja
Gao, Man
Shi, Chaonan
Jimenez-Gongora, Tamara
Fan, Pengfei
Ding, Xue
Zhang, Dan
Ding, Yi
Rosas-Díaz, Tábata
Liu, Yujing
Aguilar, Emmanuel
Fu, Xing
Lozano-Durán, Rosa
Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title_full Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title_fullStr Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title_full_unstemmed Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title_short Combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
title_sort combinatorial interactions between viral proteins expand the potential functional landscape of the tomato yellow leaf curl virus proteome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633003/
https://www.ncbi.nlm.nih.gov/pubmed/36256684
http://dx.doi.org/10.1371/journal.ppat.1010909
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