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Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines
Grapevine trunk diseases (GTDs) are a significant problem for New Zealand viticulture. Endophytic actinobacteria are of interest as potential biocontrol agents due to their ability to inhibit plant pathogens and improve plant growth. However, no studies have investigated the diversity of actinobacte...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633489/ https://www.ncbi.nlm.nih.gov/pubmed/36329295 http://dx.doi.org/10.1007/s00284-022-03068-0 |
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author | Kanjanamaneesathian, Patanun Shah, Anish Ridgway, Hayley Jones, E. Eirian |
author_facet | Kanjanamaneesathian, Patanun Shah, Anish Ridgway, Hayley Jones, E. Eirian |
author_sort | Kanjanamaneesathian, Patanun |
collection | PubMed |
description | Grapevine trunk diseases (GTDs) are a significant problem for New Zealand viticulture. Endophytic actinobacteria are of interest as potential biocontrol agents due to their ability to inhibit plant pathogens and improve plant growth. However, no studies have investigated the diversity of actinobacteria associated with grapevines in New Zealand vineyards and their bioactivity. Actinobacteria diversity in different ‘Sauvignon blanc’ vine tissues from three vineyards (conventional and organic management, and different vine ages) was assessed using different methods and media. Forty-six endophytic actinobacteria were isolated, with more isolates recovered from roots (n = 45) than leaves (n = 1) and shoot internodes (n = 0). More isolates were recovered from the organic (n = 21) than conventional (n = 8) vineyard, mature (25-year old; n = 21) than young (2-year old; n = 2) vines and using a tissue maceration technique (n = 40). Actinomycete Isolation Agar, International Streptomyces Project 2, and Starch Casein media were effective for actinobacteria isolation. Most of the isolates recovered belonged to Streptomyces, with one isolate identified as Mycolicibacterium. Forty isolates were assessed for antifungal activity and plant growth-promoting (PGP) characteristics. Of these, 13 isolates had antifungal activity against test GTD pathogens (Dactylonectria macrodidyma, Eutypa lata, Ilyonectria liriodendri, Neofusicoccum parvum, and N. luteum). Eighteen isolates exhibited more than one PGP trait; 25siderophore production (n = 25), phosphate solubilization (n = 6), and indole acetic acid production (n = 16). Two strains, Streptomyces sp. LUVPK-22 and Streptomyces sp. LUVPK-30, exhibited the best antifungal and PGP properties. This study revealed the diversity of culturable endophytic actinobacteria from grapevines in New Zealand vineyards and their biocontrol potential against GTD pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-03068-0. |
format | Online Article Text |
id | pubmed-9633489 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-96334892022-11-05 Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines Kanjanamaneesathian, Patanun Shah, Anish Ridgway, Hayley Jones, E. Eirian Curr Microbiol Article Grapevine trunk diseases (GTDs) are a significant problem for New Zealand viticulture. Endophytic actinobacteria are of interest as potential biocontrol agents due to their ability to inhibit plant pathogens and improve plant growth. However, no studies have investigated the diversity of actinobacteria associated with grapevines in New Zealand vineyards and their bioactivity. Actinobacteria diversity in different ‘Sauvignon blanc’ vine tissues from three vineyards (conventional and organic management, and different vine ages) was assessed using different methods and media. Forty-six endophytic actinobacteria were isolated, with more isolates recovered from roots (n = 45) than leaves (n = 1) and shoot internodes (n = 0). More isolates were recovered from the organic (n = 21) than conventional (n = 8) vineyard, mature (25-year old; n = 21) than young (2-year old; n = 2) vines and using a tissue maceration technique (n = 40). Actinomycete Isolation Agar, International Streptomyces Project 2, and Starch Casein media were effective for actinobacteria isolation. Most of the isolates recovered belonged to Streptomyces, with one isolate identified as Mycolicibacterium. Forty isolates were assessed for antifungal activity and plant growth-promoting (PGP) characteristics. Of these, 13 isolates had antifungal activity against test GTD pathogens (Dactylonectria macrodidyma, Eutypa lata, Ilyonectria liriodendri, Neofusicoccum parvum, and N. luteum). Eighteen isolates exhibited more than one PGP trait; 25siderophore production (n = 25), phosphate solubilization (n = 6), and indole acetic acid production (n = 16). Two strains, Streptomyces sp. LUVPK-22 and Streptomyces sp. LUVPK-30, exhibited the best antifungal and PGP properties. This study revealed the diversity of culturable endophytic actinobacteria from grapevines in New Zealand vineyards and their biocontrol potential against GTD pathogens. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-03068-0. Springer US 2022-11-03 2022 /pmc/articles/PMC9633489/ /pubmed/36329295 http://dx.doi.org/10.1007/s00284-022-03068-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kanjanamaneesathian, Patanun Shah, Anish Ridgway, Hayley Jones, E. Eirian Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title | Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title_full | Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title_fullStr | Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title_full_unstemmed | Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title_short | Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines |
title_sort | diversity and bioactivity of endophytic actinobacteria associated with grapevines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633489/ https://www.ncbi.nlm.nih.gov/pubmed/36329295 http://dx.doi.org/10.1007/s00284-022-03068-0 |
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