Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application

To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more...

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Autores principales: Sugiura, Kazunori, Nagai, Takeharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633818/
https://www.ncbi.nlm.nih.gov/pubmed/36329112
http://dx.doi.org/10.1038/s42003-022-04153-7
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author Sugiura, Kazunori
Nagai, Takeharu
author_facet Sugiura, Kazunori
Nagai, Takeharu
author_sort Sugiura, Kazunori
collection PubMed
description To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more than 10 years. Here, we report the development of Sumire, a fluorescent protein emitting 414 nm violet fluorescence from a hydrated chromophore. The Sumire’s fluorescence property allows for the creation of FRET probes that can be used simultaneously with CFP-YFP based FRET probes for multi-parameter analysis.
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spelling pubmed-96338182022-11-05 Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application Sugiura, Kazunori Nagai, Takeharu Commun Biol Article To perform correlation analysis between different physiological parameters using fluorescent protein-based functional probes, diversification of wavelength properties of fluorescent proteins is underway. However, the shortest emission wavelength of fluorescent proteins has not been updated for more than 10 years. Here, we report the development of Sumire, a fluorescent protein emitting 414 nm violet fluorescence from a hydrated chromophore. The Sumire’s fluorescence property allows for the creation of FRET probes that can be used simultaneously with CFP-YFP based FRET probes for multi-parameter analysis. Nature Publishing Group UK 2022-11-03 /pmc/articles/PMC9633818/ /pubmed/36329112 http://dx.doi.org/10.1038/s42003-022-04153-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sugiura, Kazunori
Nagai, Takeharu
Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title_full Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title_fullStr Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title_full_unstemmed Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title_short Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
title_sort extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633818/
https://www.ncbi.nlm.nih.gov/pubmed/36329112
http://dx.doi.org/10.1038/s42003-022-04153-7
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