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Chromosome-level genome assembly of the bar-headed goose (Anser indicus)

Bar-headed geese (Anser indicus) are adaptable to plateau environments. In this study, we sequenced and assembled a high-quality chromosome-level genome of the bar-headed goose using PacBio long reads and Hi-C technique, and generated 115.73 Gb of Illumina short-reads and 95.89 Gb of PacBio long-rea...

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Autores principales: Zhang, Yawen, Zhang, Bo, Zhang, Ying, Nie, Ruixue, Zhang, Jian, Shang, Peng, Zhang, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633837/
https://www.ncbi.nlm.nih.gov/pubmed/36329062
http://dx.doi.org/10.1038/s41597-022-01801-9
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author Zhang, Yawen
Zhang, Bo
Zhang, Ying
Nie, Ruixue
Zhang, Jian
Shang, Peng
Zhang, Hao
author_facet Zhang, Yawen
Zhang, Bo
Zhang, Ying
Nie, Ruixue
Zhang, Jian
Shang, Peng
Zhang, Hao
author_sort Zhang, Yawen
collection PubMed
description Bar-headed geese (Anser indicus) are adaptable to plateau environments. In this study, we sequenced and assembled a high-quality chromosome-level genome of the bar-headed goose using PacBio long reads and Hi-C technique, and generated 115.73 Gb of Illumina short-reads and 95.89 Gb of PacBio long-reads. The assembled bar-headed goose genome, with a contig N50 of 5.734 Mb and a scaffold N50 of 65.77 Mb, is 1.129 Gb in length and includes 33 chromosomes and 451 fragments. BUSCO assessment yielded a completeness score of 94.4%. In total, 15,376 protein-coding genes were predicted, of which 94.95% had homologs in protein databases. We identified 78 positively selected genes (PSGs) in the bar-headed goose genome, which were mainly enriched in calcium ion and ATP-binding. This bar-headed goose genome will be an important resource for increasing our understanding regarding the genetic basis of adaptation to life at a high altitude.
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spelling pubmed-96338372022-11-05 Chromosome-level genome assembly of the bar-headed goose (Anser indicus) Zhang, Yawen Zhang, Bo Zhang, Ying Nie, Ruixue Zhang, Jian Shang, Peng Zhang, Hao Sci Data Data Descriptor Bar-headed geese (Anser indicus) are adaptable to plateau environments. In this study, we sequenced and assembled a high-quality chromosome-level genome of the bar-headed goose using PacBio long reads and Hi-C technique, and generated 115.73 Gb of Illumina short-reads and 95.89 Gb of PacBio long-reads. The assembled bar-headed goose genome, with a contig N50 of 5.734 Mb and a scaffold N50 of 65.77 Mb, is 1.129 Gb in length and includes 33 chromosomes and 451 fragments. BUSCO assessment yielded a completeness score of 94.4%. In total, 15,376 protein-coding genes were predicted, of which 94.95% had homologs in protein databases. We identified 78 positively selected genes (PSGs) in the bar-headed goose genome, which were mainly enriched in calcium ion and ATP-binding. This bar-headed goose genome will be an important resource for increasing our understanding regarding the genetic basis of adaptation to life at a high altitude. Nature Publishing Group UK 2022-11-03 /pmc/articles/PMC9633837/ /pubmed/36329062 http://dx.doi.org/10.1038/s41597-022-01801-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Data Descriptor
Zhang, Yawen
Zhang, Bo
Zhang, Ying
Nie, Ruixue
Zhang, Jian
Shang, Peng
Zhang, Hao
Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title_full Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title_fullStr Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title_full_unstemmed Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title_short Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
title_sort chromosome-level genome assembly of the bar-headed goose (anser indicus)
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633837/
https://www.ncbi.nlm.nih.gov/pubmed/36329062
http://dx.doi.org/10.1038/s41597-022-01801-9
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