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Genetic similarity and diversity among three camel populations reared in Egypt

BACKGROUND: Molecular genetics has been extremely useful in determining the relation between animal populations and documenting the degrees of genetic variation found within them. The present study was undertaken to evaluate genetic diversity and the relationships between the three camel populations...

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Autores principales: Abdel-Aziem, Sekena H., Mabrouk, Dalia M., Abd El-Kader, Heba A., Alam, Sally S., Othman, Othman E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633884/
https://www.ncbi.nlm.nih.gov/pubmed/36326964
http://dx.doi.org/10.1186/s43141-022-00435-z
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author Abdel-Aziem, Sekena H.
Mabrouk, Dalia M.
Abd El-Kader, Heba A.
Alam, Sally S.
Othman, Othman E.
author_facet Abdel-Aziem, Sekena H.
Mabrouk, Dalia M.
Abd El-Kader, Heba A.
Alam, Sally S.
Othman, Othman E.
author_sort Abdel-Aziem, Sekena H.
collection PubMed
description BACKGROUND: Molecular genetics has been extremely useful in determining the relation between animal populations and documenting the degrees of genetic variation found within them. The present study was undertaken to evaluate genetic diversity and the relationships between the three camel populations reared in Egypt: Maghrabi, Sudani, and Baladi using mitochondrial 16S sequences and other breeds of camels in the world. METHODS: Blood samples were collected from camels belonging to these three populations. Genomic DNA was extracted from the collected blood samples and subjected to PCR using specific primers for mitochondrial 16S region. The amplified products were purified using DNA purification kit to remove residual primers and dNTPs. Sequencing was performed in the Macrogen Incorporation. The amplified products were submitted to GenBank/NCBI under accession numbers OM 278349 and OM 278350 RESULTS: Sequencing was done on the partial mitochondrial 16S amplified fragments at 530 bp. This amplified area had two haplotypes. There was one substitution (G/A) at nucleotide 309 of the amplified segment. The nucleotide (π) and Hd stand for haplotype diversity, respectively, at 0.00008 and 0.042, and the average number of pairwise nucleotide differences, k, is 0.042, according to Fu’s Fs statistic and Tajima’s D, which is −1.10686. Genetic distance percentages between the three populations under study range from 0.000 to 0.0312. A phylogenetic analysis of Egyptian camel populations and other Camelus dromedarius populations revealed a strong relationship between them. CONCLUSIONS: This study suggests that the 16S rRNA sequencing in mitochondria plays a critical role in genetic variation studies and analysis of phylogeny between camel populations and breeds.
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spelling pubmed-96338842022-11-28 Genetic similarity and diversity among three camel populations reared in Egypt Abdel-Aziem, Sekena H. Mabrouk, Dalia M. Abd El-Kader, Heba A. Alam, Sally S. Othman, Othman E. J Genet Eng Biotechnol Research BACKGROUND: Molecular genetics has been extremely useful in determining the relation between animal populations and documenting the degrees of genetic variation found within them. The present study was undertaken to evaluate genetic diversity and the relationships between the three camel populations reared in Egypt: Maghrabi, Sudani, and Baladi using mitochondrial 16S sequences and other breeds of camels in the world. METHODS: Blood samples were collected from camels belonging to these three populations. Genomic DNA was extracted from the collected blood samples and subjected to PCR using specific primers for mitochondrial 16S region. The amplified products were purified using DNA purification kit to remove residual primers and dNTPs. Sequencing was performed in the Macrogen Incorporation. The amplified products were submitted to GenBank/NCBI under accession numbers OM 278349 and OM 278350 RESULTS: Sequencing was done on the partial mitochondrial 16S amplified fragments at 530 bp. This amplified area had two haplotypes. There was one substitution (G/A) at nucleotide 309 of the amplified segment. The nucleotide (π) and Hd stand for haplotype diversity, respectively, at 0.00008 and 0.042, and the average number of pairwise nucleotide differences, k, is 0.042, according to Fu’s Fs statistic and Tajima’s D, which is −1.10686. Genetic distance percentages between the three populations under study range from 0.000 to 0.0312. A phylogenetic analysis of Egyptian camel populations and other Camelus dromedarius populations revealed a strong relationship between them. CONCLUSIONS: This study suggests that the 16S rRNA sequencing in mitochondria plays a critical role in genetic variation studies and analysis of phylogeny between camel populations and breeds. Springer Berlin Heidelberg 2022-11-03 /pmc/articles/PMC9633884/ /pubmed/36326964 http://dx.doi.org/10.1186/s43141-022-00435-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Abdel-Aziem, Sekena H.
Mabrouk, Dalia M.
Abd El-Kader, Heba A.
Alam, Sally S.
Othman, Othman E.
Genetic similarity and diversity among three camel populations reared in Egypt
title Genetic similarity and diversity among three camel populations reared in Egypt
title_full Genetic similarity and diversity among three camel populations reared in Egypt
title_fullStr Genetic similarity and diversity among three camel populations reared in Egypt
title_full_unstemmed Genetic similarity and diversity among three camel populations reared in Egypt
title_short Genetic similarity and diversity among three camel populations reared in Egypt
title_sort genetic similarity and diversity among three camel populations reared in egypt
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633884/
https://www.ncbi.nlm.nih.gov/pubmed/36326964
http://dx.doi.org/10.1186/s43141-022-00435-z
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