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Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction

Bacterial adhesion potential constitutes the transition of bacteria from the planktonic to the static phase by promoting biofilm formation, which plays a significant role in plant-microbial interaction in the agriculture industry. In present study, the adhesion potential of five soil-borne bacterial...

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Autores principales: Shaffique, Shifa, Imran, Muhammad, Wani, Shabir Hussain, Khan, Muhamad Aqil, Kang, Sang-Mo, Adhikari, Arjun, Lee, In-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634002/
https://www.ncbi.nlm.nih.gov/pubmed/36340407
http://dx.doi.org/10.3389/fpls.2022.1004331
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author Shaffique, Shifa
Imran, Muhammad
Wani, Shabir Hussain
Khan, Muhamad Aqil
Kang, Sang-Mo
Adhikari, Arjun
Lee, In-Jung
author_facet Shaffique, Shifa
Imran, Muhammad
Wani, Shabir Hussain
Khan, Muhamad Aqil
Kang, Sang-Mo
Adhikari, Arjun
Lee, In-Jung
author_sort Shaffique, Shifa
collection PubMed
description Bacterial adhesion potential constitutes the transition of bacteria from the planktonic to the static phase by promoting biofilm formation, which plays a significant role in plant-microbial interaction in the agriculture industry. In present study, the adhesion potential of five soil-borne bacterial strains belonging to different genera was studied. All bacterial strains were capable of forming colonies and biofilms of different levels of firmness on polystyrene. Significant variation was observed in hydrophobicity and motility assays. Among the five bacterial strains (SH-6, SH-8, SH-9, SH-10, and SH-19), SH-19 had a strong hydrophobic force, while SH-10 showed the most hydrophilic property. SH-6 showed great variability in motility; SH-8 had a swimming diffusion diameter of 70 mm, which was three times higher than that of SH-19. In the motility assay, SH-9 and SH-10 showed diffusion diameters of approximately 22 mm and 55 mm, respectively. Furthermore, among the five strains, four are predominately electron donors and one is electron acceptors. Overall, positive correlation was observed among Lewis acid base properties, hydrophobicity, and biofilm forming ability. However, no correlation of motility with bacterial adhesion could be found in present experimental work. Scanning electron microscopy images confirmed the adhesion potential and biofilm ability within extra polymeric substances. Research on the role of adhesion in biofilm formation of bacteria isolated from plants is potentially conducive for developing strategies such as plant–microbial interaction to mitigate the abiotic stress.
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spelling pubmed-96340022022-11-05 Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction Shaffique, Shifa Imran, Muhammad Wani, Shabir Hussain Khan, Muhamad Aqil Kang, Sang-Mo Adhikari, Arjun Lee, In-Jung Front Plant Sci Plant Science Bacterial adhesion potential constitutes the transition of bacteria from the planktonic to the static phase by promoting biofilm formation, which plays a significant role in plant-microbial interaction in the agriculture industry. In present study, the adhesion potential of five soil-borne bacterial strains belonging to different genera was studied. All bacterial strains were capable of forming colonies and biofilms of different levels of firmness on polystyrene. Significant variation was observed in hydrophobicity and motility assays. Among the five bacterial strains (SH-6, SH-8, SH-9, SH-10, and SH-19), SH-19 had a strong hydrophobic force, while SH-10 showed the most hydrophilic property. SH-6 showed great variability in motility; SH-8 had a swimming diffusion diameter of 70 mm, which was three times higher than that of SH-19. In the motility assay, SH-9 and SH-10 showed diffusion diameters of approximately 22 mm and 55 mm, respectively. Furthermore, among the five strains, four are predominately electron donors and one is electron acceptors. Overall, positive correlation was observed among Lewis acid base properties, hydrophobicity, and biofilm forming ability. However, no correlation of motility with bacterial adhesion could be found in present experimental work. Scanning electron microscopy images confirmed the adhesion potential and biofilm ability within extra polymeric substances. Research on the role of adhesion in biofilm formation of bacteria isolated from plants is potentially conducive for developing strategies such as plant–microbial interaction to mitigate the abiotic stress. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9634002/ /pubmed/36340407 http://dx.doi.org/10.3389/fpls.2022.1004331 Text en Copyright © 2022 Shaffique, Imran, Wani, Khan, Kang, Adhikari and Lee https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shaffique, Shifa
Imran, Muhammad
Wani, Shabir Hussain
Khan, Muhamad Aqil
Kang, Sang-Mo
Adhikari, Arjun
Lee, In-Jung
Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title_full Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title_fullStr Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title_full_unstemmed Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title_short Evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
title_sort evaluating the adhesive potential of the newly isolated bacterial strains in research exploitation of plant microbial interaction
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634002/
https://www.ncbi.nlm.nih.gov/pubmed/36340407
http://dx.doi.org/10.3389/fpls.2022.1004331
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