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Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study

Wheat is the staple food crop of global importance for its grain nutrient quality. Grain iron and zinc content of the wheat grain is an important quantitatively inherited trait that is influenced by the environmental factors such as drought and heat stress. Phenotypic evaluation of 295 advanced bree...

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Autores principales: Devate, Narayana Bhat, Krishna, Hari, Sunilkumar, V. P., Manjunath, Karthik Kumar, Mishra, C. N., Jain, Neelu, Singh, G. P., Singh, P. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634069/
https://www.ncbi.nlm.nih.gov/pubmed/36338980
http://dx.doi.org/10.3389/fgene.2022.1034947
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author Devate, Narayana Bhat
Krishna, Hari
Sunilkumar, V. P.
Manjunath, Karthik Kumar
Mishra, C. N.
Jain, Neelu
Singh, G. P.
Singh, P. K.
author_facet Devate, Narayana Bhat
Krishna, Hari
Sunilkumar, V. P.
Manjunath, Karthik Kumar
Mishra, C. N.
Jain, Neelu
Singh, G. P.
Singh, P. K.
author_sort Devate, Narayana Bhat
collection PubMed
description Wheat is the staple food crop of global importance for its grain nutrient quality. Grain iron and zinc content of the wheat grain is an important quantitatively inherited trait that is influenced by the environmental factors such as drought and heat stress. Phenotypic evaluation of 295 advanced breeding lines from the wheat stress breeding program of IARI was carried out under timely sown irrigated (IR), restricted irrigated, and late-sown conditions at New Delhi during the cropping season of 2020–21, and grain iron (GFeC) and zinc (GZnC) contents were estimated from both control and treatments. A statistically significant increase in GFeC and GZnC was observed under stress conditions compared to that of the control. Genotyping was carried out with the SNPs from the 35K Axiom Breeder’s array, and marker–trait association was identified by GWAS analysis. Of the 23 MTAs identified, seven were linked with GFeC and sixteen were linked with GZnC. In silico analysis revealed a few important transcripts involved in various plant metabolism, growth, and development activities such as auxin response factor, root UVB sensitive proteins, potassium transporter, glycosyl transferase, COBRA, and F-box-like domain. The identified MTAs can be used for molecular breeding after validation and also for rapid development of micronutrient-rich varieties of wheat to mitigate hidden hunger.
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spelling pubmed-96340692022-11-05 Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study Devate, Narayana Bhat Krishna, Hari Sunilkumar, V. P. Manjunath, Karthik Kumar Mishra, C. N. Jain, Neelu Singh, G. P. Singh, P. K. Front Genet Genetics Wheat is the staple food crop of global importance for its grain nutrient quality. Grain iron and zinc content of the wheat grain is an important quantitatively inherited trait that is influenced by the environmental factors such as drought and heat stress. Phenotypic evaluation of 295 advanced breeding lines from the wheat stress breeding program of IARI was carried out under timely sown irrigated (IR), restricted irrigated, and late-sown conditions at New Delhi during the cropping season of 2020–21, and grain iron (GFeC) and zinc (GZnC) contents were estimated from both control and treatments. A statistically significant increase in GFeC and GZnC was observed under stress conditions compared to that of the control. Genotyping was carried out with the SNPs from the 35K Axiom Breeder’s array, and marker–trait association was identified by GWAS analysis. Of the 23 MTAs identified, seven were linked with GFeC and sixteen were linked with GZnC. In silico analysis revealed a few important transcripts involved in various plant metabolism, growth, and development activities such as auxin response factor, root UVB sensitive proteins, potassium transporter, glycosyl transferase, COBRA, and F-box-like domain. The identified MTAs can be used for molecular breeding after validation and also for rapid development of micronutrient-rich varieties of wheat to mitigate hidden hunger. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9634069/ /pubmed/36338980 http://dx.doi.org/10.3389/fgene.2022.1034947 Text en Copyright © 2022 Devate, Krishna, Sunilkumar, Manjunath, Mishra, Jain, Singh and Singh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Devate, Narayana Bhat
Krishna, Hari
Sunilkumar, V. P.
Manjunath, Karthik Kumar
Mishra, C. N.
Jain, Neelu
Singh, G. P.
Singh, P. K.
Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title_full Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title_fullStr Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title_full_unstemmed Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title_short Identification of genomic regions of wheat associated with grain Fe and Zn content under drought and heat stress using genome-wide association study
title_sort identification of genomic regions of wheat associated with grain fe and zn content under drought and heat stress using genome-wide association study
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634069/
https://www.ncbi.nlm.nih.gov/pubmed/36338980
http://dx.doi.org/10.3389/fgene.2022.1034947
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