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Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill

Occurrence of multidrug resistant Enterobacteriaceae in livestock is of concern as they can spread to humans. A potential introduction route for these bacteria to livestock could be animal feed. We therefore wanted to identify if Escherichia spp., Enterobacter spp., Klebsiella spp., or Raoutella spp...

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Autores principales: Börjesson, Stefan, Brouwer, Michael S. M., Östlund, Emma, Eriksson, Jenny, Elving, Josefine, Karlsson Lindsjö, Oskar, Engblom, Linda I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634252/
https://www.ncbi.nlm.nih.gov/pubmed/36338068
http://dx.doi.org/10.3389/fmicb.2022.993454
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author Börjesson, Stefan
Brouwer, Michael S. M.
Östlund, Emma
Eriksson, Jenny
Elving, Josefine
Karlsson Lindsjö, Oskar
Engblom, Linda I.
author_facet Börjesson, Stefan
Brouwer, Michael S. M.
Östlund, Emma
Eriksson, Jenny
Elving, Josefine
Karlsson Lindsjö, Oskar
Engblom, Linda I.
author_sort Börjesson, Stefan
collection PubMed
description Occurrence of multidrug resistant Enterobacteriaceae in livestock is of concern as they can spread to humans. A potential introduction route for these bacteria to livestock could be animal feed. We therefore wanted to identify if Escherichia spp., Enterobacter spp., Klebsiella spp., or Raoutella spp. with transferable resistance to extended spectrum cephalosporins, carbapenems or colistin could be detected in the environment at feed mills in Sweden. A second aim was to compare detected isolates to previous described isolates from humans and animals in Sweden to establish relatedness which could indicate a potential transmission between sectors and feed mills as a source for antibiotic resistant bacteria. However, no isolates with transferable resistance to extended-cephalosporins or colistin could be identified, but one isolate belonging to the Enterobacter cloacae complex was shown to be carbapenem-resistant and showing carbapenemase-activity. Based on sequencing by both short-read Illumina and long-read Oxford Nanopore MinIon technologies it was shown that this isolate was an E. asburiae carrying a bla(IMI-2) gene on a 216 Kbp plasmid, designated pSB89A/IMI-2, and contained the plasmid replicons IncFII, IncFIB, and a third replicon showing highest similarity to the IncFII(Yp). In addition, the plasmid contained genes for various functions such as plasmid segregation and stability, plasmid transfer and arsenical transport, but no additional antibiotic resistance genes. This isolate and the pSB89A/IMI-2 was compared to three human clinical isolates positive for bla(IMI-2) available from the Swedish antibiotic monitoring program Swedres. It was shown that one of the human isolates carried a plasmid similar with regards to gene content to the pSB89A/IMI-2 except for the plasmid transfer system, but that the order of genes was different. The pSB89A/IMI-2 did however share the same transfer system as the bla(IMI-2) carrying plasmids from the other two human isolates. The pSB89A/IMI-2 was also compared to previously published plasmids carrying bla(IMI-2), but no identical plasmids could be identified. However, most shared part of the plasmid transfer system and DNA replication genes, and the bla(IMI-2) gene was located next the transcription regulator imiR. The IS3-family insertion element downstream of imiR in the pSB89A was also related to the IS elements in other bla(IMI)-carrying plasmids.
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spelling pubmed-96342522022-11-05 Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill Börjesson, Stefan Brouwer, Michael S. M. Östlund, Emma Eriksson, Jenny Elving, Josefine Karlsson Lindsjö, Oskar Engblom, Linda I. Front Microbiol Microbiology Occurrence of multidrug resistant Enterobacteriaceae in livestock is of concern as they can spread to humans. A potential introduction route for these bacteria to livestock could be animal feed. We therefore wanted to identify if Escherichia spp., Enterobacter spp., Klebsiella spp., or Raoutella spp. with transferable resistance to extended spectrum cephalosporins, carbapenems or colistin could be detected in the environment at feed mills in Sweden. A second aim was to compare detected isolates to previous described isolates from humans and animals in Sweden to establish relatedness which could indicate a potential transmission between sectors and feed mills as a source for antibiotic resistant bacteria. However, no isolates with transferable resistance to extended-cephalosporins or colistin could be identified, but one isolate belonging to the Enterobacter cloacae complex was shown to be carbapenem-resistant and showing carbapenemase-activity. Based on sequencing by both short-read Illumina and long-read Oxford Nanopore MinIon technologies it was shown that this isolate was an E. asburiae carrying a bla(IMI-2) gene on a 216 Kbp plasmid, designated pSB89A/IMI-2, and contained the plasmid replicons IncFII, IncFIB, and a third replicon showing highest similarity to the IncFII(Yp). In addition, the plasmid contained genes for various functions such as plasmid segregation and stability, plasmid transfer and arsenical transport, but no additional antibiotic resistance genes. This isolate and the pSB89A/IMI-2 was compared to three human clinical isolates positive for bla(IMI-2) available from the Swedish antibiotic monitoring program Swedres. It was shown that one of the human isolates carried a plasmid similar with regards to gene content to the pSB89A/IMI-2 except for the plasmid transfer system, but that the order of genes was different. The pSB89A/IMI-2 did however share the same transfer system as the bla(IMI-2) carrying plasmids from the other two human isolates. The pSB89A/IMI-2 was also compared to previously published plasmids carrying bla(IMI-2), but no identical plasmids could be identified. However, most shared part of the plasmid transfer system and DNA replication genes, and the bla(IMI-2) gene was located next the transcription regulator imiR. The IS3-family insertion element downstream of imiR in the pSB89A was also related to the IS elements in other bla(IMI)-carrying plasmids. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9634252/ /pubmed/36338068 http://dx.doi.org/10.3389/fmicb.2022.993454 Text en Copyright © 2022 Börjesson, Brouwer, Östlund, Eriksson, Elving, Karlsson Lindsjö and Engblom. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Börjesson, Stefan
Brouwer, Michael S. M.
Östlund, Emma
Eriksson, Jenny
Elving, Josefine
Karlsson Lindsjö, Oskar
Engblom, Linda I.
Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title_full Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title_fullStr Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title_full_unstemmed Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title_short Detection of an IMI-2 carbapenemase-producing Enterobacter asburiae at a Swedish feed mill
title_sort detection of an imi-2 carbapenemase-producing enterobacter asburiae at a swedish feed mill
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634252/
https://www.ncbi.nlm.nih.gov/pubmed/36338068
http://dx.doi.org/10.3389/fmicb.2022.993454
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