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Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet

Tibetan pigs were thought to have good performances of rough feeding tolerance, which may be related to the gut microbiota. This study was conducted to investigate the changes of colonic microbiota contribute to fiber utilization in Tibetan pigs fed alfalfa supplementation diet compared with basal d...

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Autores principales: Gao, Qingtao, Sun, Guangming, Duan, Jiujun, Luo, Chengzeng, Yangji, Cidan, Zhong, Ruqing, Chen, Liang, Zhu, Yanbin, Wangdui, Basang, Zhang, Hongfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634421/
https://www.ncbi.nlm.nih.gov/pubmed/36338094
http://dx.doi.org/10.3389/fmicb.2022.969524
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author Gao, Qingtao
Sun, Guangming
Duan, Jiujun
Luo, Chengzeng
Yangji, Cidan
Zhong, Ruqing
Chen, Liang
Zhu, Yanbin
Wangdui, Basang
Zhang, Hongfu
author_facet Gao, Qingtao
Sun, Guangming
Duan, Jiujun
Luo, Chengzeng
Yangji, Cidan
Zhong, Ruqing
Chen, Liang
Zhu, Yanbin
Wangdui, Basang
Zhang, Hongfu
author_sort Gao, Qingtao
collection PubMed
description Tibetan pigs were thought to have good performances of rough feeding tolerance, which may be related to the gut microbiota. This study was conducted to investigate the changes of colonic microbiota contribute to fiber utilization in Tibetan pigs fed alfalfa supplementation diet compared with basal diet, and verified whether the microbial community in Tibetan pigs fed alfalfa diet was beneficial to utilize fiber using in vitro fermentation. A total of 40 Tibetan pigs were allocated into two groups and fed with a corn-soybean meal basal diet (CD) or a 50% alfalfa supplementation diet (AD) for 42d. Our results showed pigs fed CD diet improved carcass weight compared to pigs fed AD diet (p < 0.05), yet reduced the bacterial diversity (p < 0.05). Tibetan pigs fed CD diet increased certain pathogenic bacteria (Streptococcus) abundance (FDR < 0.05). Alfalfa consumption increased fiber-degrading bacteria abundance (UCG-005, Rikenellaceae_RC9_gut_group, Prevotellaceae_UCG-003, Alloprevotella, Marvinbryantia, and Anaerovibrio) in the colonic digesta (FDR < 0.05) and improved concentrations of acetate, propionate, butyrate, and total SCFA in colonic content (p < 0.05). Higher fermentation capacity of fecal microbiota from pig fed AD diet was verified by in vitro fermentation. Collectively, our results indicated that alfalfa supplementation in diets improved the abundance of fiber-degrading bacteria and SCFA production in the hindgut of Tibetan pig, as well as enhanced the fermentation capacity of fecal microbiota.
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spelling pubmed-96344212022-11-05 Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet Gao, Qingtao Sun, Guangming Duan, Jiujun Luo, Chengzeng Yangji, Cidan Zhong, Ruqing Chen, Liang Zhu, Yanbin Wangdui, Basang Zhang, Hongfu Front Microbiol Microbiology Tibetan pigs were thought to have good performances of rough feeding tolerance, which may be related to the gut microbiota. This study was conducted to investigate the changes of colonic microbiota contribute to fiber utilization in Tibetan pigs fed alfalfa supplementation diet compared with basal diet, and verified whether the microbial community in Tibetan pigs fed alfalfa diet was beneficial to utilize fiber using in vitro fermentation. A total of 40 Tibetan pigs were allocated into two groups and fed with a corn-soybean meal basal diet (CD) or a 50% alfalfa supplementation diet (AD) for 42d. Our results showed pigs fed CD diet improved carcass weight compared to pigs fed AD diet (p < 0.05), yet reduced the bacterial diversity (p < 0.05). Tibetan pigs fed CD diet increased certain pathogenic bacteria (Streptococcus) abundance (FDR < 0.05). Alfalfa consumption increased fiber-degrading bacteria abundance (UCG-005, Rikenellaceae_RC9_gut_group, Prevotellaceae_UCG-003, Alloprevotella, Marvinbryantia, and Anaerovibrio) in the colonic digesta (FDR < 0.05) and improved concentrations of acetate, propionate, butyrate, and total SCFA in colonic content (p < 0.05). Higher fermentation capacity of fecal microbiota from pig fed AD diet was verified by in vitro fermentation. Collectively, our results indicated that alfalfa supplementation in diets improved the abundance of fiber-degrading bacteria and SCFA production in the hindgut of Tibetan pig, as well as enhanced the fermentation capacity of fecal microbiota. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9634421/ /pubmed/36338094 http://dx.doi.org/10.3389/fmicb.2022.969524 Text en Copyright © 2022 Gao, Sun, Duan, Luo, Yangji, Zhong, Chen, Zhu, Wangdui and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gao, Qingtao
Sun, Guangming
Duan, Jiujun
Luo, Chengzeng
Yangji, Cidan
Zhong, Ruqing
Chen, Liang
Zhu, Yanbin
Wangdui, Basang
Zhang, Hongfu
Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title_full Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title_fullStr Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title_full_unstemmed Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title_short Alterations in gut microbiota improve SCFA production and fiber utilization in Tibetan pigs fed alfalfa diet
title_sort alterations in gut microbiota improve scfa production and fiber utilization in tibetan pigs fed alfalfa diet
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634421/
https://www.ncbi.nlm.nih.gov/pubmed/36338094
http://dx.doi.org/10.3389/fmicb.2022.969524
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