Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster

We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pk (pk1) or a premature stop codon in the second exon of pk (sple1) . Additional likely benign missense mutations were identified in both mutant isoforms.