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Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634456/ https://www.ncbi.nlm.nih.gov/pubmed/36338150 http://dx.doi.org/10.17912/micropub.biology.000656 |
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author | Lilienthal, Anthony J Parida, Mrutyunjaya Manak, J Robert |
author_facet | Lilienthal, Anthony J Parida, Mrutyunjaya Manak, J Robert |
author_sort | Lilienthal, Anthony J |
collection | PubMed |
description | We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pk (pk1) or a premature stop codon in the second exon of pk (sple1) . Additional likely benign missense mutations were identified in both mutant isoforms. |
format | Online Article Text |
id | pubmed-9634456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-96344562022-11-05 Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster Lilienthal, Anthony J Parida, Mrutyunjaya Manak, J Robert MicroPubl Biol New Finding We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pk (pk1) or a premature stop codon in the second exon of pk (sple1) . Additional likely benign missense mutations were identified in both mutant isoforms. Caltech Library 2022-10-20 /pmc/articles/PMC9634456/ /pubmed/36338150 http://dx.doi.org/10.17912/micropub.biology.000656 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Lilienthal, Anthony J Parida, Mrutyunjaya Manak, J Robert Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster |
title |
Characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
Drosophila melanogaster
|
title_full |
Characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
Drosophila melanogaster
|
title_fullStr |
Characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
Drosophila melanogaster
|
title_full_unstemmed |
Characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
Drosophila melanogaster
|
title_short |
Characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
Drosophila melanogaster
|
title_sort | characterization of
prickle
isoform-specific
pk
(pk1)
and
pk
(sple1)
mutations in
drosophila melanogaster |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634456/ https://www.ncbi.nlm.nih.gov/pubmed/36338150 http://dx.doi.org/10.17912/micropub.biology.000656 |
work_keys_str_mv | AT lilienthalanthonyj characterizationofprickleisoformspecificpkpk1andpksple1mutationsindrosophilamelanogaster AT paridamrutyunjaya characterizationofprickleisoformspecificpkpk1andpksple1mutationsindrosophilamelanogaster AT manakjrobert characterizationofprickleisoformspecificpkpk1andpksple1mutationsindrosophilamelanogaster |