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Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster

We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertio...

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Detalles Bibliográficos
Autores principales: Lilienthal, Anthony J, Parida, Mrutyunjaya, Manak, J Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634456/
https://www.ncbi.nlm.nih.gov/pubmed/36338150
http://dx.doi.org/10.17912/micropub.biology.000656
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author Lilienthal, Anthony J
Parida, Mrutyunjaya
Manak, J Robert
author_facet Lilienthal, Anthony J
Parida, Mrutyunjaya
Manak, J Robert
author_sort Lilienthal, Anthony J
collection PubMed
description We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pk (pk1) or a premature stop codon in the second exon of pk (sple1) . Additional likely benign missense mutations were identified in both mutant isoforms.
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spelling pubmed-96344562022-11-05 Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster Lilienthal, Anthony J Parida, Mrutyunjaya Manak, J Robert MicroPubl Biol New Finding We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pk (pk1) and pk (sple1) mutations of the prickle gene in Drosophila melanogaster . Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pk (pk1) or a premature stop codon in the second exon of pk (sple1) . Additional likely benign missense mutations were identified in both mutant isoforms. Caltech Library 2022-10-20 /pmc/articles/PMC9634456/ /pubmed/36338150 http://dx.doi.org/10.17912/micropub.biology.000656 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Lilienthal, Anthony J
Parida, Mrutyunjaya
Manak, J Robert
Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title_full Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title_fullStr Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title_full_unstemmed Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title_short Characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in Drosophila melanogaster
title_sort characterization of prickle isoform-specific pk (pk1) and pk (sple1) mutations in drosophila melanogaster
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634456/
https://www.ncbi.nlm.nih.gov/pubmed/36338150
http://dx.doi.org/10.17912/micropub.biology.000656
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