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VaporSPOT: Parallel Synthesis of Oligosaccharides on Membranes

[Image: see text] Automated chemical synthesis has revolutionized synthetic access to biopolymers in terms of simplicity and speed. While automated oligosaccharide synthesis has become faster and more versatile, the parallel synthesis of oligosaccharides is not yet possible. Here, a chemical vapor g...

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Detalles Bibliográficos
Autores principales: Tsouka, Alexandra, Dallabernardina, Pietro, Mende, Marco, Sletten, Eric T., Leichnitz, Sabrina, Bienert, Klaus, Le Mai Hoang, Kim, Seeberger, Peter H., Loeffler, Felix F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634802/
https://www.ncbi.nlm.nih.gov/pubmed/36269942
http://dx.doi.org/10.1021/jacs.2c07285
Descripción
Sumario:[Image: see text] Automated chemical synthesis has revolutionized synthetic access to biopolymers in terms of simplicity and speed. While automated oligosaccharide synthesis has become faster and more versatile, the parallel synthesis of oligosaccharides is not yet possible. Here, a chemical vapor glycosylation strategy (VaporSPOT) is described that enables the simultaneous synthesis of oligosaccharides on a cellulose membrane solid support. Different linkers allow for flexible and straightforward cleavage, purification, and characterization of the target oligosaccharides. This method is the basis for the development of parallel automated glycan synthesis platforms.