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Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease

BACKGROUND: Macrophages are key innate immune cells implicated in the pathogenesis of Behçet’s disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. MET...

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Autores principales: Wu, Xiuhua, Wang, Zhimian, Shi, Jing, Yu, Xin, Li, Chaoran, Liu, Jinjing, Zhang, Fengchun, Chen, Hua, Zheng, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635113/
https://www.ncbi.nlm.nih.gov/pubmed/36333776
http://dx.doi.org/10.1186/s13075-022-02938-z
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author Wu, Xiuhua
Wang, Zhimian
Shi, Jing
Yu, Xin
Li, Chaoran
Liu, Jinjing
Zhang, Fengchun
Chen, Hua
Zheng, Wenjie
author_facet Wu, Xiuhua
Wang, Zhimian
Shi, Jing
Yu, Xin
Li, Chaoran
Liu, Jinjing
Zhang, Fengchun
Chen, Hua
Zheng, Wenjie
author_sort Wu, Xiuhua
collection PubMed
description BACKGROUND: Macrophages are key innate immune cells implicated in the pathogenesis of Behçet’s disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. METHODS: BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4(+)T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum. RESULTS: BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-α and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages and were enriched in NF-κB signaling. BD serum-treated macrophages showed upregulated p-p65 and downregulated IκBα, and NF-κB inhibitor attenuated BD serum-stimulated M1-like phenotype. CONCLUSIONS: BD serum promoted macrophage polarization toward a proinflammatory M1-like phenotype through NF-κB signaling and potentially facilitated inflammation in BD. M1 polarized macrophages may be a potential therapeutic target for BD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13075-022-02938-z.
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spelling pubmed-96351132022-11-05 Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease Wu, Xiuhua Wang, Zhimian Shi, Jing Yu, Xin Li, Chaoran Liu, Jinjing Zhang, Fengchun Chen, Hua Zheng, Wenjie Arthritis Res Ther Research BACKGROUND: Macrophages are key innate immune cells implicated in the pathogenesis of Behçet’s disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization. METHODS: BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4(+)T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum. RESULTS: BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-α and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages and were enriched in NF-κB signaling. BD serum-treated macrophages showed upregulated p-p65 and downregulated IκBα, and NF-κB inhibitor attenuated BD serum-stimulated M1-like phenotype. CONCLUSIONS: BD serum promoted macrophage polarization toward a proinflammatory M1-like phenotype through NF-κB signaling and potentially facilitated inflammation in BD. M1 polarized macrophages may be a potential therapeutic target for BD. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13075-022-02938-z. BioMed Central 2022-11-04 2022 /pmc/articles/PMC9635113/ /pubmed/36333776 http://dx.doi.org/10.1186/s13075-022-02938-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wu, Xiuhua
Wang, Zhimian
Shi, Jing
Yu, Xin
Li, Chaoran
Liu, Jinjing
Zhang, Fengchun
Chen, Hua
Zheng, Wenjie
Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title_full Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title_fullStr Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title_full_unstemmed Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title_short Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease
title_sort macrophage polarization toward m1 phenotype through nf-κb signaling in patients with behçet’s disease
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635113/
https://www.ncbi.nlm.nih.gov/pubmed/36333776
http://dx.doi.org/10.1186/s13075-022-02938-z
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