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Raman Spectroscopy for Chemical Biology Research
[Image: see text] In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there are limitations to this technology, such as the limited number of colors that can be detected simultaneously. Recen...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635364/ https://www.ncbi.nlm.nih.gov/pubmed/36216344 http://dx.doi.org/10.1021/jacs.2c05359 |
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author | Dodo, Kosuke Fujita, Katsumasa Sodeoka, Mikiko |
author_facet | Dodo, Kosuke Fujita, Katsumasa Sodeoka, Mikiko |
author_sort | Dodo, Kosuke |
collection | PubMed |
description | [Image: see text] In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there are limitations to this technology, such as the limited number of colors that can be detected simultaneously. Recently, Raman spectroscopy has been applied in chemical biology to overcome such limitations. Raman spectroscopy detects the molecular vibrations reflecting the structures and chemical conditions of molecules in a sample and was originally used to directly visualize the chemical responses of endogenous molecules. However, our initial research to develop “Raman tags” opens a new avenue for the application of Raman spectroscopy in chemical biology. In this Perspective, we first introduce the label-free Raman imaging of biomolecules, illustrating the biological applications of Raman spectroscopy. Next, we highlight the application of Raman imaging of small molecules using Raman tags for chemical biology research. Finally, we discuss the development and potential of Raman probes, which represent the next-generation probes in chemical biology. |
format | Online Article Text |
id | pubmed-9635364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-96353642023-10-10 Raman Spectroscopy for Chemical Biology Research Dodo, Kosuke Fujita, Katsumasa Sodeoka, Mikiko J Am Chem Soc [Image: see text] In chemical biology research, various fluorescent probes have been developed and used to visualize target proteins or molecules in living cells and tissues, yet there are limitations to this technology, such as the limited number of colors that can be detected simultaneously. Recently, Raman spectroscopy has been applied in chemical biology to overcome such limitations. Raman spectroscopy detects the molecular vibrations reflecting the structures and chemical conditions of molecules in a sample and was originally used to directly visualize the chemical responses of endogenous molecules. However, our initial research to develop “Raman tags” opens a new avenue for the application of Raman spectroscopy in chemical biology. In this Perspective, we first introduce the label-free Raman imaging of biomolecules, illustrating the biological applications of Raman spectroscopy. Next, we highlight the application of Raman imaging of small molecules using Raman tags for chemical biology research. Finally, we discuss the development and potential of Raman probes, which represent the next-generation probes in chemical biology. American Chemical Society 2022-10-10 2022-11-02 /pmc/articles/PMC9635364/ /pubmed/36216344 http://dx.doi.org/10.1021/jacs.2c05359 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Dodo, Kosuke Fujita, Katsumasa Sodeoka, Mikiko Raman Spectroscopy for Chemical Biology Research |
title | Raman
Spectroscopy for Chemical Biology Research |
title_full | Raman
Spectroscopy for Chemical Biology Research |
title_fullStr | Raman
Spectroscopy for Chemical Biology Research |
title_full_unstemmed | Raman
Spectroscopy for Chemical Biology Research |
title_short | Raman
Spectroscopy for Chemical Biology Research |
title_sort | raman
spectroscopy for chemical biology research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635364/ https://www.ncbi.nlm.nih.gov/pubmed/36216344 http://dx.doi.org/10.1021/jacs.2c05359 |
work_keys_str_mv | AT dodokosuke ramanspectroscopyforchemicalbiologyresearch AT fujitakatsumasa ramanspectroscopyforchemicalbiologyresearch AT sodeokamikiko ramanspectroscopyforchemicalbiologyresearch |