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SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans

RNA interference is sequence-specific gene silencing triggered by double-stranded RNA. Systemic RNA interference is where double-stranded RNA, expressed or introduced into 1 cell, is transported to and initiates RNA interference in other cells. Systemic RNA interference is very efficient in Caenorha...

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Autores principales: Bhatia, Sonya, Hunter, Craig P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635667/
https://www.ncbi.nlm.nih.gov/pubmed/36165710
http://dx.doi.org/10.1093/g3journal/jkac252
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author Bhatia, Sonya
Hunter, Craig P
author_facet Bhatia, Sonya
Hunter, Craig P
author_sort Bhatia, Sonya
collection PubMed
description RNA interference is sequence-specific gene silencing triggered by double-stranded RNA. Systemic RNA interference is where double-stranded RNA, expressed or introduced into 1 cell, is transported to and initiates RNA interference in other cells. Systemic RNA interference is very efficient in Caenorhabditis elegans and genetic screens for systemic RNA interference-defective mutants have identified RNA transporters (SID-1, SID-2, and SID-5) and a signaling protein (SID-3). Here, we report that SID-4 is nck-1, a C. elegans NCK-like adaptor protein. sid-4 null mutations cause a weak, dose-sensitive, systemic RNA interference defect and can be effectively rescued by SID-4 expression in target tissues only, implying a role in double-stranded RNA import. SID-4 and SID-3 (ACK-1 kinase) homologs interact in mammals and insects, suggesting that they may function in a common signaling pathway; however, a sid-3; sid-4 double mutants showed additive resistance to RNA interference, suggesting that these proteins likely interact with other signaling pathways as well. A bioinformatic screen coupled to RNA interference sensitivity tests identified 23 additional signaling components with weak RNA interference-defective phenotypes. These observations suggest that environmental conditions may modulate systemic RNA interference efficacy, and indeed, sid-3 and sid-4 are required for growth temperature effects on systemic RNA interference silencing efficiency.
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spelling pubmed-96356672022-11-07 SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans Bhatia, Sonya Hunter, Craig P G3 (Bethesda) Investigation RNA interference is sequence-specific gene silencing triggered by double-stranded RNA. Systemic RNA interference is where double-stranded RNA, expressed or introduced into 1 cell, is transported to and initiates RNA interference in other cells. Systemic RNA interference is very efficient in Caenorhabditis elegans and genetic screens for systemic RNA interference-defective mutants have identified RNA transporters (SID-1, SID-2, and SID-5) and a signaling protein (SID-3). Here, we report that SID-4 is nck-1, a C. elegans NCK-like adaptor protein. sid-4 null mutations cause a weak, dose-sensitive, systemic RNA interference defect and can be effectively rescued by SID-4 expression in target tissues only, implying a role in double-stranded RNA import. SID-4 and SID-3 (ACK-1 kinase) homologs interact in mammals and insects, suggesting that they may function in a common signaling pathway; however, a sid-3; sid-4 double mutants showed additive resistance to RNA interference, suggesting that these proteins likely interact with other signaling pathways as well. A bioinformatic screen coupled to RNA interference sensitivity tests identified 23 additional signaling components with weak RNA interference-defective phenotypes. These observations suggest that environmental conditions may modulate systemic RNA interference efficacy, and indeed, sid-3 and sid-4 are required for growth temperature effects on systemic RNA interference silencing efficiency. Oxford University Press 2022-09-27 /pmc/articles/PMC9635667/ /pubmed/36165710 http://dx.doi.org/10.1093/g3journal/jkac252 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Bhatia, Sonya
Hunter, Craig P
SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title_full SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title_fullStr SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title_full_unstemmed SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title_short SID-4/NCK-1 is important for dsRNA import in Caenorhabditis elegans
title_sort sid-4/nck-1 is important for dsrna import in caenorhabditis elegans
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635667/
https://www.ncbi.nlm.nih.gov/pubmed/36165710
http://dx.doi.org/10.1093/g3journal/jkac252
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