Cargando…

Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions

Engineered living materials (ELMs) are gaining traction among synthetic biologists, as their emergent properties and nonequilibrium thermodynamics make them markedly different from traditional materials. However, the aspiration to directly use living cells as building blocks to create higher-order s...

Descripción completa

Detalles Bibliográficos
Autores principales: Yi, Qikun, Dai, Xin, Park, Byung Min, Gu, Junhao, Luo, Jiren, Wang, Ri, Yu, Cong, Kou, Songzi, Huang, Jinqing, Lakerveld, Richard, Sun, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635822/
https://www.ncbi.nlm.nih.gov/pubmed/36332017
http://dx.doi.org/10.1126/sciadv.ade0073
_version_ 1784824795839332352
author Yi, Qikun
Dai, Xin
Park, Byung Min
Gu, Junhao
Luo, Jiren
Wang, Ri
Yu, Cong
Kou, Songzi
Huang, Jinqing
Lakerveld, Richard
Sun, Fei
author_facet Yi, Qikun
Dai, Xin
Park, Byung Min
Gu, Junhao
Luo, Jiren
Wang, Ri
Yu, Cong
Kou, Songzi
Huang, Jinqing
Lakerveld, Richard
Sun, Fei
author_sort Yi, Qikun
collection PubMed
description Engineered living materials (ELMs) are gaining traction among synthetic biologists, as their emergent properties and nonequilibrium thermodynamics make them markedly different from traditional materials. However, the aspiration to directly use living cells as building blocks to create higher-order structures or materials, with no need for chemical modification, remains elusive to synthetic biologists. Here, we report a strategy that enables the assembly of engineered Saccharomyces cerevisiae into self-propagating ELMs via ultrahigh-affinity protein/protein interactions. These yeast cells have been genetically engineered to display the protein pairs SpyTag/SpyCatcher or CL7/Im7 on their surfaces, which enable their assembly into multicellular structures capable of further growth and proliferation. The assembly process can be controlled precisely via optical tweezers or microfluidics. Moreover, incorporation of functional motifs such as super uranyl-binding protein and mussel foot proteins via genetic programming rendered these materials suitable for uranium extraction from seawater and bioadhesion, respectively, pointing to their potential in chemical separation and biomedical applications.
format Online
Article
Text
id pubmed-9635822
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Association for the Advancement of Science
record_format MEDLINE/PubMed
spelling pubmed-96358222022-11-18 Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions Yi, Qikun Dai, Xin Park, Byung Min Gu, Junhao Luo, Jiren Wang, Ri Yu, Cong Kou, Songzi Huang, Jinqing Lakerveld, Richard Sun, Fei Sci Adv Biomedicine and Life Sciences Engineered living materials (ELMs) are gaining traction among synthetic biologists, as their emergent properties and nonequilibrium thermodynamics make them markedly different from traditional materials. However, the aspiration to directly use living cells as building blocks to create higher-order structures or materials, with no need for chemical modification, remains elusive to synthetic biologists. Here, we report a strategy that enables the assembly of engineered Saccharomyces cerevisiae into self-propagating ELMs via ultrahigh-affinity protein/protein interactions. These yeast cells have been genetically engineered to display the protein pairs SpyTag/SpyCatcher or CL7/Im7 on their surfaces, which enable their assembly into multicellular structures capable of further growth and proliferation. The assembly process can be controlled precisely via optical tweezers or microfluidics. Moreover, incorporation of functional motifs such as super uranyl-binding protein and mussel foot proteins via genetic programming rendered these materials suitable for uranium extraction from seawater and bioadhesion, respectively, pointing to their potential in chemical separation and biomedical applications. American Association for the Advancement of Science 2022-11-04 /pmc/articles/PMC9635822/ /pubmed/36332017 http://dx.doi.org/10.1126/sciadv.ade0073 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Yi, Qikun
Dai, Xin
Park, Byung Min
Gu, Junhao
Luo, Jiren
Wang, Ri
Yu, Cong
Kou, Songzi
Huang, Jinqing
Lakerveld, Richard
Sun, Fei
Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title_full Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title_fullStr Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title_full_unstemmed Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title_short Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
title_sort directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635822/
https://www.ncbi.nlm.nih.gov/pubmed/36332017
http://dx.doi.org/10.1126/sciadv.ade0073
work_keys_str_mv AT yiqikun directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT daixin directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT parkbyungmin directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT gujunhao directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT luojiren directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT wangri directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT yucong directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT kousongzi directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT huangjinqing directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT lakerveldrichard directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions
AT sunfei directedassemblyofgeneticallyengineeredeukaryoticcellsintolivingfunctionalmaterialsviaultrahighaffinityproteininteractions