Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex

Camphorataimide B (CamB) has anticancer activities against several tumors. Here, we aimed to investigate the mechanism(s) by which CamB inhibits metastasis of colorectal cancer (CRC) cells. Low-dose CamB did not affect the cell viability and cell cycle progression of CRC cells, but significantly inh...

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Autores principales: Huang, Chi-Chou, Hung, Chia-Hung, Lee, Yi-Ju, Tseng, Tsui-Hwa, Lee, Yean-Jang, Kao, Shao-Hsuan, Wang, Chau-Jong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635903/
http://dx.doi.org/10.38212/2224-6614.3405
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author Huang, Chi-Chou
Hung, Chia-Hung
Lee, Yi-Ju
Tseng, Tsui-Hwa
Lee, Yean-Jang
Kao, Shao-Hsuan
Wang, Chau-Jong
author_facet Huang, Chi-Chou
Hung, Chia-Hung
Lee, Yi-Ju
Tseng, Tsui-Hwa
Lee, Yean-Jang
Kao, Shao-Hsuan
Wang, Chau-Jong
author_sort Huang, Chi-Chou
collection PubMed
description Camphorataimide B (CamB) has anticancer activities against several tumors. Here, we aimed to investigate the mechanism(s) by which CamB inhibits metastasis of colorectal cancer (CRC) cells. Low-dose CamB did not affect the cell viability and cell cycle progression of CRC cells, but significantly inhibited the metastatic potentials of CRC cells. Mechanically, CamB reduced the protein and mRNA expression of BMP4, and inhibited Smad and FAK/Src/Akt signaling. CamB also decreased Snail levels by promoting its degradation via proteasome and thereafter reduced Snail-mediated BMP4 transcription. Moreover, CamB considerably inhibited the in vivo metastasis of DLD-1 cells in xenograft mice.
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spelling pubmed-96359032022-12-06 Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex Huang, Chi-Chou Hung, Chia-Hung Lee, Yi-Ju Tseng, Tsui-Hwa Lee, Yean-Jang Kao, Shao-Hsuan Wang, Chau-Jong J Food Drug Anal Original Article Camphorataimide B (CamB) has anticancer activities against several tumors. Here, we aimed to investigate the mechanism(s) by which CamB inhibits metastasis of colorectal cancer (CRC) cells. Low-dose CamB did not affect the cell viability and cell cycle progression of CRC cells, but significantly inhibited the metastatic potentials of CRC cells. Mechanically, CamB reduced the protein and mRNA expression of BMP4, and inhibited Smad and FAK/Src/Akt signaling. CamB also decreased Snail levels by promoting its degradation via proteasome and thereafter reduced Snail-mediated BMP4 transcription. Moreover, CamB considerably inhibited the in vivo metastasis of DLD-1 cells in xenograft mice. Taiwan Food and Drug Administration 2022-06-15 /pmc/articles/PMC9635903/ http://dx.doi.org/10.38212/2224-6614.3405 Text en © 2022 Taiwan Food and Drug Administration https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Original Article
Huang, Chi-Chou
Hung, Chia-Hung
Lee, Yi-Ju
Tseng, Tsui-Hwa
Lee, Yean-Jang
Kao, Shao-Hsuan
Wang, Chau-Jong
Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title_full Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title_fullStr Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title_full_unstemmed Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title_short Camphorataimide B suppresses the metastasis of human colorectal cancer cell by inhibiting Smad/FAK/Akt axis and promoting degradation of Snail/BMP4 complex
title_sort camphorataimide b suppresses the metastasis of human colorectal cancer cell by inhibiting smad/fak/akt axis and promoting degradation of snail/bmp4 complex
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635903/
http://dx.doi.org/10.38212/2224-6614.3405
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