Cargando…

Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp

Dysregulated innate and adaptive immune response to rhinoviral infection plays an important role in the exacerbation or progressive course of chronic rhinosinusitis (CRS). However, few studies have evaluated whether rhinovirus-induced production of anti-viral interferon is deficient or delayed in in...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Sang Hag, Han, Mun Soo, Lee, Tae Hoon, Lee, Da Bin, Park, Jae Hyung, Lee, Seung Hyeok, Kim, Tae Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635927/
https://www.ncbi.nlm.nih.gov/pubmed/36341332
http://dx.doi.org/10.3389/fimmu.2022.1025796
_version_ 1784824823089725440
author Lee, Sang Hag
Han, Mun Soo
Lee, Tae Hoon
Lee, Da Bin
Park, Jae Hyung
Lee, Seung Hyeok
Kim, Tae Hoon
author_facet Lee, Sang Hag
Han, Mun Soo
Lee, Tae Hoon
Lee, Da Bin
Park, Jae Hyung
Lee, Seung Hyeok
Kim, Tae Hoon
author_sort Lee, Sang Hag
collection PubMed
description Dysregulated innate and adaptive immune response to rhinoviral infection plays an important role in the exacerbation or progressive course of chronic rhinosinusitis (CRS). However, few studies have evaluated whether rhinovirus-induced production of anti-viral interferon is deficient or delayed in inflammatory epithelial cells of patients with CRS with nasal polyps. The aim of the present study is to investigate the replication rates of rhinovirus 16 (RV 16), RV16-induced antiviral interferon secretion, and the expression levels of pattern recognition receptors after RV 16 infection or TLR3 stimulation with poly (I: C) in normal and inflammatory epithelial cells. Inflammatory epithelial cells were obtained from CRS patients with nasal polyps and normal epithelial cells were derived from ethmoid sinus mucosa during endoscopic reduction of blowout fracture or uncinate process mucosa of patients with septal deviation. Cultured cells were infected with RV 16 or treated with poly (I: C) for 24, 48, and 72 h. Cells and media were harvested at each time point and used to evaluate RV16 replication rates, the secretion of IFN-β, -λ1, -λ2, viperin, Mx, and OAS, and the expression levels of TRL3, RIG-I, MDA5, phospho-NFκB, and phospho-IRF3. RV replication rates reached peak levels 48 h after inoculation in both normal and inflammatory epithelial cells and showed no difference between both groups of epithelial cells at any time point. The release of IFN-β, -λ1, and -λ2 in normal and inflammatory epithelial cells was also strongly induced 48 h after RV16 inoculation but reached peak levels 24 h after poly (I: C) treatment. The expression levels of viperin, Mx, OAS, TLR3, RIG-I, MDA5, phospho-NFκB, and phospho-IRF3 showed similar patterns in both groups of epithelial cells. These results suggest that the production of RV16-induced antiviral interferons is not deficient or delayed in inflammatory epithelial cells from CRS patients with nasal polyps.
format Online
Article
Text
id pubmed-9635927
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96359272022-11-05 Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp Lee, Sang Hag Han, Mun Soo Lee, Tae Hoon Lee, Da Bin Park, Jae Hyung Lee, Seung Hyeok Kim, Tae Hoon Front Immunol Immunology Dysregulated innate and adaptive immune response to rhinoviral infection plays an important role in the exacerbation or progressive course of chronic rhinosinusitis (CRS). However, few studies have evaluated whether rhinovirus-induced production of anti-viral interferon is deficient or delayed in inflammatory epithelial cells of patients with CRS with nasal polyps. The aim of the present study is to investigate the replication rates of rhinovirus 16 (RV 16), RV16-induced antiviral interferon secretion, and the expression levels of pattern recognition receptors after RV 16 infection or TLR3 stimulation with poly (I: C) in normal and inflammatory epithelial cells. Inflammatory epithelial cells were obtained from CRS patients with nasal polyps and normal epithelial cells were derived from ethmoid sinus mucosa during endoscopic reduction of blowout fracture or uncinate process mucosa of patients with septal deviation. Cultured cells were infected with RV 16 or treated with poly (I: C) for 24, 48, and 72 h. Cells and media were harvested at each time point and used to evaluate RV16 replication rates, the secretion of IFN-β, -λ1, -λ2, viperin, Mx, and OAS, and the expression levels of TRL3, RIG-I, MDA5, phospho-NFκB, and phospho-IRF3. RV replication rates reached peak levels 48 h after inoculation in both normal and inflammatory epithelial cells and showed no difference between both groups of epithelial cells at any time point. The release of IFN-β, -λ1, and -λ2 in normal and inflammatory epithelial cells was also strongly induced 48 h after RV16 inoculation but reached peak levels 24 h after poly (I: C) treatment. The expression levels of viperin, Mx, OAS, TLR3, RIG-I, MDA5, phospho-NFκB, and phospho-IRF3 showed similar patterns in both groups of epithelial cells. These results suggest that the production of RV16-induced antiviral interferons is not deficient or delayed in inflammatory epithelial cells from CRS patients with nasal polyps. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9635927/ /pubmed/36341332 http://dx.doi.org/10.3389/fimmu.2022.1025796 Text en Copyright © 2022 Lee, Han, Lee, Lee, Park, Lee and Kim https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Lee, Sang Hag
Han, Mun Soo
Lee, Tae Hoon
Lee, Da Bin
Park, Jae Hyung
Lee, Seung Hyeok
Kim, Tae Hoon
Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title_full Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title_fullStr Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title_full_unstemmed Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title_short Rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
title_sort rhinovirus-induced anti-viral interferon secretion is not deficient and not delayed in sinonasal epithelial cells of patients with chronic rhinosinusitis with nasal polyp
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9635927/
https://www.ncbi.nlm.nih.gov/pubmed/36341332
http://dx.doi.org/10.3389/fimmu.2022.1025796
work_keys_str_mv AT leesanghag rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT hanmunsoo rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT leetaehoon rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT leedabin rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT parkjaehyung rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT leeseunghyeok rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp
AT kimtaehoon rhinovirusinducedantiviralinterferonsecretionisnotdeficientandnotdelayedinsinonasalepithelialcellsofpatientswithchronicrhinosinusitiswithnasalpolyp