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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation

Whereas techniques to map chromatin-bound proteins are well developed, mapping chromatin-associated RNAs remains a challenge. Here, we describe Reverse Transcribe and Tagment (RT&Tag), in which RNAs associated with a chromatin epitope are targeted by an antibody followed by a protein A-Tn5 trans...

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Detalles Bibliográficos
Autores principales: Khyzha, Nadiya, Henikoff, Steven, Ahmad, Kami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636022/
https://www.ncbi.nlm.nih.gov/pubmed/36192462
http://dx.doi.org/10.1038/s41592-022-01618-9

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