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Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation
Whereas techniques to map chromatin-bound proteins are well developed, mapping chromatin-associated RNAs remains a challenge. Here, we describe Reverse Transcribe and Tagment (RT&Tag), in which RNAs associated with a chromatin epitope are targeted by an antibody followed by a protein A-Tn5 trans...
Autores principales: | Khyzha, Nadiya, Henikoff, Steven, Ahmad, Kami |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636022/ https://www.ncbi.nlm.nih.gov/pubmed/36192462 http://dx.doi.org/10.1038/s41592-022-01618-9 |
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