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Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation
Protein synthesis is an essential step in gene expression during the development of mammalian preimplantation embryos. This is a complex and highly regulated process. The accuracy of the translation initiation codon is important in various gene expression programs. However, the mechanisms that regul...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636173/ https://www.ncbi.nlm.nih.gov/pubmed/36333315 http://dx.doi.org/10.1038/s41467-022-34427-x |
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author | Zhang, Jue Pi, Shuai-Bo Zhang, Nan Guo, Jing Zheng, Wei Leng, Lizhi Lin, Ge Fan, Heng-Yu |
author_facet | Zhang, Jue Pi, Shuai-Bo Zhang, Nan Guo, Jing Zheng, Wei Leng, Lizhi Lin, Ge Fan, Heng-Yu |
author_sort | Zhang, Jue |
collection | PubMed |
description | Protein synthesis is an essential step in gene expression during the development of mammalian preimplantation embryos. This is a complex and highly regulated process. The accuracy of the translation initiation codon is important in various gene expression programs. However, the mechanisms that regulate AUG and non-AUG codon initiation in early embryos remain poorly understood. BZW1 is a key factor in determining the mRNA translation start codon. Here, we show that BZW1 is essential for early embryonic development in mice. Bzw1-knockdown embryos fail to undergo compaction, and show decreased blastocyst formation rates. We also observe defects in the differentiation capacity and implantation potential after Bzw1 interference. Further investigation revealed that Bzw1 knockdown causes the levels of translation initiation with CUG as the start codon to increase. The decline in BZW1 levels result in a decrease in protein synthesis in preimplantation embryos, whereas the total mRNA levels are not altered. Therefore, we concluded that BZW1 contributes to protein synthesis during early embryonic development by restricting non-AUG translational initiation. |
format | Online Article Text |
id | pubmed-9636173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96361732022-11-06 Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation Zhang, Jue Pi, Shuai-Bo Zhang, Nan Guo, Jing Zheng, Wei Leng, Lizhi Lin, Ge Fan, Heng-Yu Nat Commun Article Protein synthesis is an essential step in gene expression during the development of mammalian preimplantation embryos. This is a complex and highly regulated process. The accuracy of the translation initiation codon is important in various gene expression programs. However, the mechanisms that regulate AUG and non-AUG codon initiation in early embryos remain poorly understood. BZW1 is a key factor in determining the mRNA translation start codon. Here, we show that BZW1 is essential for early embryonic development in mice. Bzw1-knockdown embryos fail to undergo compaction, and show decreased blastocyst formation rates. We also observe defects in the differentiation capacity and implantation potential after Bzw1 interference. Further investigation revealed that Bzw1 knockdown causes the levels of translation initiation with CUG as the start codon to increase. The decline in BZW1 levels result in a decrease in protein synthesis in preimplantation embryos, whereas the total mRNA levels are not altered. Therefore, we concluded that BZW1 contributes to protein synthesis during early embryonic development by restricting non-AUG translational initiation. Nature Publishing Group UK 2022-11-04 /pmc/articles/PMC9636173/ /pubmed/36333315 http://dx.doi.org/10.1038/s41467-022-34427-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Jue Pi, Shuai-Bo Zhang, Nan Guo, Jing Zheng, Wei Leng, Lizhi Lin, Ge Fan, Heng-Yu Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title | Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title_full | Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title_fullStr | Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title_full_unstemmed | Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title_short | Translation regulatory factor BZW1 regulates preimplantation embryo development and compaction by restricting global non-AUG Initiation |
title_sort | translation regulatory factor bzw1 regulates preimplantation embryo development and compaction by restricting global non-aug initiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636173/ https://www.ncbi.nlm.nih.gov/pubmed/36333315 http://dx.doi.org/10.1038/s41467-022-34427-x |
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