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Identification of evolutionarily conserved regulators of muscle mitochondrial network organization

Mitochondrial networks provide coordinated energy distribution throughout muscle cells. However, pathways specifying mitochondrial networks are incompletely understood and it is unclear how they might affect contractile fiber-type. Here, we show that natural energetic demands placed on Drosophila me...

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Autores principales: Katti, Prasanna, Ajayi, Peter T., Aponte, Angel, Bleck, Christopher K. E., Glancy, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636386/
https://www.ncbi.nlm.nih.gov/pubmed/36333356
http://dx.doi.org/10.1038/s41467-022-34445-9
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author Katti, Prasanna
Ajayi, Peter T.
Aponte, Angel
Bleck, Christopher K. E.
Glancy, Brian
author_facet Katti, Prasanna
Ajayi, Peter T.
Aponte, Angel
Bleck, Christopher K. E.
Glancy, Brian
author_sort Katti, Prasanna
collection PubMed
description Mitochondrial networks provide coordinated energy distribution throughout muscle cells. However, pathways specifying mitochondrial networks are incompletely understood and it is unclear how they might affect contractile fiber-type. Here, we show that natural energetic demands placed on Drosophila melanogaster muscles yield native cell-types among which contractile and mitochondrial network-types are regulated differentially. Proteomic analyses of indirect flight, jump, and leg muscles, together with muscles misexpressing known fiber-type specification factor salm, identified transcription factors H15 and cut as potential mitochondrial network regulators. We demonstrate H15 operates downstream of salm regulating flight muscle contractile and mitochondrial network-type. Conversely, H15 regulates mitochondrial network configuration but not contractile type in jump and leg muscles. Further, we find that cut regulates salm expression in flight muscles and mitochondrial network configuration in leg muscles. These data indicate cell type-specific regulation of muscle mitochondrial network organization through evolutionarily conserved transcription factors cut, salm, and H15.
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spelling pubmed-96363862022-11-06 Identification of evolutionarily conserved regulators of muscle mitochondrial network organization Katti, Prasanna Ajayi, Peter T. Aponte, Angel Bleck, Christopher K. E. Glancy, Brian Nat Commun Article Mitochondrial networks provide coordinated energy distribution throughout muscle cells. However, pathways specifying mitochondrial networks are incompletely understood and it is unclear how they might affect contractile fiber-type. Here, we show that natural energetic demands placed on Drosophila melanogaster muscles yield native cell-types among which contractile and mitochondrial network-types are regulated differentially. Proteomic analyses of indirect flight, jump, and leg muscles, together with muscles misexpressing known fiber-type specification factor salm, identified transcription factors H15 and cut as potential mitochondrial network regulators. We demonstrate H15 operates downstream of salm regulating flight muscle contractile and mitochondrial network-type. Conversely, H15 regulates mitochondrial network configuration but not contractile type in jump and leg muscles. Further, we find that cut regulates salm expression in flight muscles and mitochondrial network configuration in leg muscles. These data indicate cell type-specific regulation of muscle mitochondrial network organization through evolutionarily conserved transcription factors cut, salm, and H15. Nature Publishing Group UK 2022-11-04 /pmc/articles/PMC9636386/ /pubmed/36333356 http://dx.doi.org/10.1038/s41467-022-34445-9 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Katti, Prasanna
Ajayi, Peter T.
Aponte, Angel
Bleck, Christopher K. E.
Glancy, Brian
Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title_full Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title_fullStr Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title_full_unstemmed Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title_short Identification of evolutionarily conserved regulators of muscle mitochondrial network organization
title_sort identification of evolutionarily conserved regulators of muscle mitochondrial network organization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636386/
https://www.ncbi.nlm.nih.gov/pubmed/36333356
http://dx.doi.org/10.1038/s41467-022-34445-9
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